if you make up a solution of 100mL of 0.1M HEPES in the basic form, what will be the pH?
HEPES is a weak acid. The pKa value of this acid is 7.5. The pKb of HEPES can be calculated as given below
pKa + pKb = 14
pKb = 14- pKa = 14 - 7.5
pKb =14 - 7.5
= 6.5
Now calculate the Ka value of HEPES as
Kb = -log(pKb)
= -log(6.5)
= 3.16 x 10-7
As the solution is prepared in basic condition having concentration of 0.1 M. Thus, the [OH-] of weak base can be calculated as
[OH-] = (Kb x c)1/2
= [(3.16 x 10-7)(0.1M)]1/2
= 1.78 x 10-4M
Using above value, calculate the pOH value as
pOH = -log(OH-)
= -log(1.78 x 10-4M)
= 3.75
Now calculate the pH of solution as
pH = 14 - pOH
pH = 14 - 3.75
pH = 10.25
Thus, the pH of the solution will be 10.25.
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