Another similar problem to the one posted earlier, but with a different volume... • I count...
Another similar problem to the one posted earlier, but with a different volume...
• I count 176 colonies on a plate onto which 100 ml of a 10^-5 dilution of my culture was plated.
•what was the concentration of my original culture?
Homework Answers
So, this problem needs calculation of CFU/ml first.
Cfu/ml = Number of colonies/Vomume of sample plated*dilution factor
= 176/100*105 = 176/107 = 176*10-7 or 1.76*10-9 cfu/ml
To get the concentration of original sample, the cfu/ml has to be mutiplied with the dilution factor.
So, it becomes = 1.76*10-9 cfu/ml * 105 = 1.76*10-2 cfu/ml
Therefore, 1.76*10-2 cfu/ml is the concentration of original sample.
Hope it helps, Good luck !!!!
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