Design an experiment that will allow you to follow a protein from the endoplasmic reticulum, Golgi...
Design an experiment that will allow you to follow a protein from the endoplasmic reticulum, Golgi complex, and secretion vesicles to the outside of the cell. How will this differ if the protein is localized within lysosomes?
Homework Answers
Experiment:
*We should insert an GFP ( green fluorescent protein in the gene which encodes protein by means of recombinant DNA technology.
* We should also take normal cell as control.
* Now look in the microscope, you will find the green fluorescent in the Endoplasmic reticulum whent The movement of protein occurs along the Endoplasmic reticulum.
* For surity, compare it with wild type control cell.
If the protein is localised to lysosome then the green fluorescent is seen in lysosome only. You will not found these greenish in any other organelles of cell except lysosome
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Design an experiment that will allow you to follow a protein
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2. You are trying to design a protein that will be expressed in Escherichia coli and...
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purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
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You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system
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Chapter 4: A Tour of the Cell 5. Smooth endoplasmic reticulum (SER) lacks _, which gives it a "smooth" appearance. 6. True or false: If false, make it a correct statement. The smooth endoplasmic reticulum con- tains many enzymes that allow it perform functions like detoxify circulating drugs. 7. Use Figures 4.14 (page 65 of your textbook) and 4.17 (page 67 of your textbook) to answer the following question. Place the following steps in the order in which they occur...
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2. You are trying to design a protein that will be expressed
in Escherichia coli and secreted outside of the cell for
purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
cell. Which secretion system would work best for this project?
Support your answer with evidence based on the properties of E.
coli, the protein, and the secretion system. (20 pts)
2. You are trying to design a...
Match the organelle with its function. 14 01:04:36 A. Instructions for protein synthesis and cell reproduction contains genetic information B. Intracellular compartment forms transport vesicles, participates in lipid synthesis and synthesis of membrane or secreted proteins lekto rach Click to select) C. Packages proteins for export from cell; forms secretory vesicle Click to select D. Digest wom-out organelles and cell debris: digest material taken up by endocytosis cick to select E. "Power plants of the cell; sites of oxidative metabolism...
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