Question

Can someone help me answers these? These questions can have one answer, more than one answer, or none.

Which of the following factors directly affects the distance and speed of a migration of a protein during electrophoresis?
- affinity of protein to agarose/polysaccharides
- solubility of protein
- shape of protein
- function of a protein
- size of protein
(I chose the size of protein, and I got partial wrong 2/4)

What is the function of sodium dodecyl sulfate (SDS) in SDS-PAGE?
-to introduce pores into the gel
-none of the above
-to normalize protein charges and allow separation based solely on size
-to unfold proteins
-to sanitize proteins and get rid of cytosolic residues
( I chose to normalize protein charges and allow separation based solely on size and I got it partially correct. 2/4pts)

Isoelectric focusing allows separation based on:
-size
-none of the above
-shape
-net charge
-pI
(I chose pI and I got it partially correct. 2/4)

Two-dimensional electrophoresis allows separation based on:
-melting temperature
-none of the above
-solubility
-size
-isoelectric point
( I chose none of the above and I got it incorrect.)

  

  

Answer 1