Homework Answers
2) Km represents the complexation efficiency of a substrate with enzyme, thus it is in a better way also called as binding constant or binding efficiency.
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__________ In a mixture of 4 proteins listed below, which one should elute the last in...
15. In which of the following procedures does the sample stop moving until conditions change? i....
15. In which of the following procedures does the sample stop moving until conditions change? i. Size exclusion chromatography ii. SDS-electrophoresis iii. Isoelectric Focusing iv. Density gradient centrifugation V. Rate-zonal centrifugation a. ii, iii, and v. b. iii, and iv. c. ii, iv, and v. d. only iii e. all excepti. 16. or the following types of separation, which gives one the best chance of obtaining a protein in its properly folded state? a. Affinity Chromatography b. SDS Electrophoresis c....
Which technique could be used for molecular weight determinations in proteins under all circumstances? a.) gel...
Which technique could be used for molecular weight determinations in proteins under all circumstances? a.) gel filtration b.) isoelectric focusing c.) carboxymethyl (CM) cellulose chromatography d.) a and e e.) SDS-PAGE
Which of these 5 proteins will elute first from the gel filtration (size exclusion) column? Serum albumin (68 kDa) a-la...
Which of these 5 proteins will elute first from the gel filtration (size exclusion) column? Serum albumin (68 kDa) a-lactalbumin (14.2 kDa) O Lactoferrin (87 kDa) k-casein (19 kDa) O b-lactoglobulin (18 kDa) O Glucagon activation of a G protein-coupled membrane receptor (GPCR) induces GTP binding to which of the following membrane anchored proleins? Gbg Phospdlipase A2 None of the mentioned options The GPCR itself Ga Which of the following sphingolipids is a phospholipid? Sphingomyelin Gangliosides None of the mentioned...
Can someone answer the question and provide a short explanation? Thanks! Which of the following statements...
Can someone answer the question and provide a short explanation? Thanks! Which of the following statements is NOT correct regarding protein seperation techniques? A) Size-exclusion chromatography works on the same principle as polyacrylamide gel electrophoresis B) Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation C) Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography D) Western blotting is a useful technique to isolate proteins for use in downstream...
32. Which of the following would make a protein of a large size move faster through...
32. Which of the following would make a protein of a large size move faster through an SDS gel electrophoresis separation? a. add more protein to your sample b. add more buffer c. remove some buffer d. decrease the acrylamide concentration of the gel e. increase the acrylamide concentration of the gel 33. Which of the following statements are accurate? i. SDS binds to proteins, introducing a negative charge which is proportional to the length of the protein primary structure....
a. Which spot corresponds to the protein with the largest molecular weight? b. When pH = 7, what order would the follow...
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
Which method would work best to isolate protein Z from this mixture of proteins? Protein M.W. ...
Which method would work best to isolate protein Z from this mixture of proteins? Protein M.W. pI X 20,000 6.1 Y 75,000 9.1 Z 110,000 4.6 Answer choices: a. cation exchange at pH=7 b. size-exclusion P100 gel (5,000-100,000 MW fractionation range) c. anion exchange at pH=7 d. size-exclusion P60 gel (3,000-60,000 MW fractionation range)
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity...
14. Recombinant MBP-, GST- or GFP- fusion proteins are expressed in bacteria not only for affinity chromatography but also to ___________. A. overexpress the proteins in bacteria B. to reduce toxicity of the foreign proteins C. for proper folding and keeping the proteins soluble D. to trace the cytological location of the foreign proteins 15. You have constructed an insulin-GST fusion protein and expressed it in E. coli. You want to separate the recombinant...
19, Which of the following is true? Enrymes force reactions to proceed in only one direction....
19, Which of the following is true? Enrymes force reactions to proceed in only one direction. A) Enrymes alter the equilibrium of the reaction. Enzymes alter the standard free energy of the reaction. All of the answers are correct. None of the answers is correct B) C) D) E) 20). When substrate concentration is much greater than K, the rate of catalysis is almost equal to A) K B) /K C) Vea D) All of the answers are correct. E)...
15. In which of the following procedures does the sample stop moving until conditions change? i. Size exclusion chromatography ii. SDS-electrophoresis iii. Isoelectric Focusing iv. Density gradient centrifugation V. Rate-zonal centrifugation a. ii, iii, and v. b. iii, and iv. c. ii, iv, and v. d. only iii e. all excepti. 16. or the following types of separation, which gives one the best chance of obtaining a protein in its properly folded state? a. Affinity Chromatography b. SDS Electrophoresis c....
Which of these 5 proteins will elute first from the gel filtration (size exclusion) column? Serum albumin (68 kDa) a-lactalbumin (14.2 kDa) O Lactoferrin (87 kDa) k-casein (19 kDa) O b-lactoglobulin (18 kDa) O Glucagon activation of a G protein-coupled membrane receptor (GPCR) induces GTP binding to which of the following membrane anchored proleins? Gbg Phospdlipase A2 None of the mentioned options The GPCR itself Ga Which of the following sphingolipids is a phospholipid? Sphingomyelin Gangliosides None of the mentioned...
32. Which of the following would make a protein of a large size move faster through an SDS gel electrophoresis separation? a. add more protein to your sample b. add more buffer c. remove some buffer d. decrease the acrylamide concentration of the gel e. increase the acrylamide concentration of the gel 33. Which of the following statements are accurate? i. SDS binds to proteins, introducing a negative charge which is proportional to the length of the protein primary structure....
a. Which spot corresponds to the protein with the largest
molecular weight?
b. When pH = 7, what order would the following peptides elute
from a column filled with cation exchange resin? Size exclusion
column?
Protein 1 – 400 kDa;
10%A 5%E, 5%F, 10% G, 5% H, 10%I, 10%K, 10%L 10%R, 5%P, 5%S, 10%W,
5%Y
Protein 2: 40 kDa
5%A, 5%C, 5%D, 5%E, 5%F, 10%G, 5%I, 10%K, 5%L, 5%M, 5%N, 10%P, 5%R,
5%S, 5%T, 5%V, 5%W,
Protein 3 – 175...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
19, Which of the following is true? Enrymes force reactions to proceed in only one direction. A) Enrymes alter the equilibrium of the reaction. Enzymes alter the standard free energy of the reaction. All of the answers are correct. None of the answers is correct B) C) D) E) 20). When substrate concentration is much greater than K, the rate of catalysis is almost equal to A) K B) /K C) Vea D) All of the answers are correct. E)...
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