The E. coli strain H104:O4 caused the 2011 outbreak in Germany. This strain encodes several virulence factors that were acquired through horizontal gene transfer. If you are given the complete genome sequence of this strain, how can you identify the genomic regions containing these horizontally transferred virulence genes?
The E. coli strain H104:O4 caused the 2011 outbreak in Germany. This strain encodes several virulence...
The CDC has determined that the E. coli O157:H7 strain that caused the multistate outbreak associated with redleaf and greenleaf lettuce was the same strain that caused an outbreak in 2017, which was linked to leafy greens in the US and romain lettuce in Canada; but it was not the same strain that caused the outbreak in 2018 that was linked to romaine lettuce grown in Yuma, AZ. What method do epidemiologists at the CDC and local public health laboratories...
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second job at a hip new microbrew (you make a great IPA). You find that this strain ferments more efficiently and adds a superior flavour profile to your brews. You want to clone the strain of yeast and generate a genomic library to determine the genes responsible for this finding. To do so you: 1. Obtain fragments of the whole yeast genome (DNA) through restriction...
Brucellosis, caused by gram-negative Brucella species, is a zoonotic disease with serious impact on the livestock industry. In animals, brucellosis can lead to abortions in females and sterility in males. In humans, brucellosis is rarely fatal, but it does cause systemic febrile (fever-causing) disease that can be debilitating. Brucella bacteria enter macrophages but are able to evade phagolysosomal fusion and so are able to survive intracellularly. Brucella, unlike other gramnegative bacteria, such as E. coli, has an unusual LPS that...
Brucellosis, caused by gram-negative Brucella species, is a zoonotic disease with serious impact on the livestock industry. In animals, brucellosis can lead to abortions in females and sterility in males. In humans, brucellosis is rarely fatal, but it does cause systemic febrile (fever-causing) disease that can be debilitating. Brucella bacteria enter macrophages but are able to evade phagolysosomal fusion and so are able to survive intracellularly. Brucella, unlike other gramnegative bacteria, such as E. coli, has an unusual LPS that...