Question

What is the purpose Pfaffl equation ? what does the result of the equation tells us about gene expression levels?
Explain the background of pfaffl equation.

Answer 1

Pfaffl equation is used to calculate relative gene expression data while accounting for differences in primer efficiencies.

Plaffl formula

Gene Expression ratio = (E_GOI​​​​​​) ^{delta Ct GOI}/(E_HKG​​​​​​) ^{delta Ct HKG}

^{Where E refers to the primer efficiency}

^{GOI refers to gene of interest}

^{HKG refers to house keeping gene}

^{Ct means cycle threshold.}

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