What is the oxidized state of ABTS molecules by incubating ABTS with H2O2? Explain your answer...

Question

Question

What is the oxidized state of ABTS molecules by incubating ABTS with H2O2? Explain your answer using redox potential gradients.

Answer 1

Answer #1

Standard reduction potential of H₂O₂ is 1.763 V

H₂O₂ + 2H⁺ + 2e^- -> 2H₂O E^o = 1.776V vs SHE

Standard reduction potentials of ABTS are as follows

ABTS⁺ + e^- -> ABTS E^o = 0.67 V vs SHE

ABTS²⁺ + e^- -> ABTS⁺ E^o = 1.09 V vs SHE

Therefore redox potential of ABTS -> ABTS²⁺ +2e^- is 1.76 V < redox potential for H₂O₂

Therefore, H₂O₂ will oxidize ABTS to ABTS⁺

Answer 2

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