What is happening molecularly that causes primers to not bind as well at high temperatures?
What is happening molecularly that causes primers to not bind as well at high temperatures?
Homework Answers
The high temperature causes the hydrogen bonds between the bases in two strands of template DNA to break and the two strands to separate.The bacteria's DNA polymerase is very stable at high temperatures, which means it can withstand the temperatures needed to break the strands of DNA apart in the denaturing stage of PCR. DNA polymerase from most other organisms would not be able to withstand these high temperatures, for example, human polymerase works ideally at 37˚C.
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What is happening molecularly that causes primers to not bind as
well at high temperatures?
In high-throughput sequencing, the primers bind to a) oligonucleotides attached to the fragments. b) free dNTPs....
In high-throughput sequencing, the primers bind to a) oligonucleotides attached to the fragments. b) free dNTPs. c) DNA polymerase. d) the sequence fragments themselves. e) nothing.
Question 3 1 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if...
Question 3 1 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any, the reaction will be spontaneous. A. AS rxn= +100 J/K; AH rxn= +150 kJ [ Select] B. AS rxn= - 100 J/K; AH rxn= +200 kJ [Select]
Question 5 1 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if...
Question 5 1 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any, the reaction will be spontaneous. A. 4NO(g)+6H2O(g) 4NH3(g)+502(g) AHrxn=+906kJ [Select] B. 2N2O(g)+O2(g)-4NO(g) AH rxn=+197.1kJ [Select] C. C3H3(g)+502(g)-3CO2(g)+4H2O(g) AH rxn=-2044kJ Spontaneous at all temperatura A
3. (4 pts.) Describe what is happening (with a brief why as well) at the origin...
3. (4 pts.) Describe what is happening (with a brief why as well) at the origin on c = = g(x, y) with the level curve graph: C-3 CS-2 CE- CO-1 C=-2 C=-3 0
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Question 6 1.5 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any, the following reactions will be spontaneous. A. 2CO(g) + O2(g) = 2002(g), AH rxn=-566.0 kJ [ Select] B. 2NO2(g)2NO(g)+O2(g), AH rxn= +113.1 kJ [ Select ] C. 2H2(g)+O2(g)—2H2O(g), AH rxn=-483.6 kJ [ Select] D. 2H2O2(1)→2H2O(1)+O2(g), AH rxn= -196.0 kJ [Select ]
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Question 3 1 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any, the reaction will be spontaneous. A. AS rxn= +100 J/K; AH rxn= +150 kJ [ Select] B. AS rxn= - 100 J/K; AH rxn= +200 kJ [Select]
Question 5 1 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any, the reaction will be spontaneous. A. 4NO(g)+6H2O(g) 4NH3(g)+502(g) AHrxn=+906kJ [Select] B. 2N2O(g)+O2(g)-4NO(g) AH rxn=+197.1kJ [Select] C. C3H3(g)+502(g)-3CO2(g)+4H2O(g) AH rxn=-2044kJ Spontaneous at all temperatura A
3. (4 pts.) Describe what is happening (with a brief why as well) at the origin on c = = g(x, y) with the level curve graph: C-3 CS-2 CE- CO-1 C=-2 C=-3 0
Question 6 1.5 pts Predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any, the following reactions will be spontaneous. A. 2CO(g) + O2(g) = 2002(g), AH rxn=-566.0 kJ [ Select] B. 2NO2(g)2NO(g)+O2(g), AH rxn= +113.1 kJ [ Select ] C. 2H2(g)+O2(g)—2H2O(g), AH rxn=-483.6 kJ [ Select] D. 2H2O2(1)→2H2O(1)+O2(g), AH rxn= -196.0 kJ [Select ]
Exercise 18.38-Enhanced-with Feedback 7 of 13> Part E ReviewI ConstantsI Periodic Table In addition, predict under what temperatures (all temperatures, low temperatures, or high temperatures), if any the reaction in each part will be spontaneous. Without doing any calculations, iddentify the signs of ΔSays and ΔSsurr for each of the following chemical reactions. Drag the appropriate items to their respective bins. Reset Help Reaction is spontaneous at all temperatures Reaction is spontaneous at low temperatures Reaction is spontaneous at high...
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