Please help. this question is confusing How ninhydrin can be used to measure protease activity and...
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Please help. this question is confusing
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How ninhydrin can be used to measure protease activity and Why did we measure A620 with the spectrophotometer and what would we be detecting if we had measured A550?
Homework Answers
Answer:
Ninhydrin is a chemical compound which reacts with amines to produce a chromophoric compound. We can measure protease activity using ninhydrin as follows.
Ninhydrin reacts with products of protease enzyme, and leads to formation of colored compound. The intensity of color, tells about the activity level of protease enzyme.
Protease + Substrate 
Coloress
product
Ninhydrin + Colorless product Colored
compound (ninhydrin-product complex)
We measure absorbance at 620 nm because the nihydrin - product complex has maximum absorption at 620 nm.
At 550 nm, we would be detecting for cytochrome c .
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