Question

Explain how you would identify a tissue on an unlabeled slide (How would you confirm or eliminate any of the primary tissues on the slide?)

Answer 1

Ans . There are four basic type of tissue which includes epithelium, connective tissue, nervous tissue and muscles. Each contain there sub type that may look different but have some similar characteristics.

1) Epithelium

Characteristics to identify-

a) look for a free apical surface - every epithelial tissue has a free surface that face either a hollow organ' s inner surface or outer environment ( skin).

b) determine the number of cell layer - an epithelial tissue is divided into two sub classes on the basis of number of layers - 1) simple epithelium ( single layer); 2) compound epithelium ( more than two layers)

c) determine the apical cell shape - on the basis of cell shape of apical layer epithelium is divided into three type - 1) squamous epithelium (flat cells); 2) cuboidal epithelium ( cuboidal cells) 3) columnar epithelium ( columnar cells)

NOTE - if the single layer of epithelium which consists of two type of cells some small and some are large due to which it look like multi layer. This type of tissue are called pseudostratified epithelium.

2) Connective tissue - marked by presence of matrix. Further divided into areolar connective tissue, adipose tissue, cartilage, bone and blood

a) No free surface ( no apical surface)

b) fibers are irregularly arranged, space present in between fibers (named areolae) then it is areolar connective tissue.

c) if the middle space is filled with fat globule ( either single large or small, many) than it is adipose connective tissue.

d) Cartilage - marked by presence of extensive matrix, fibers and a space present in center named lacuna ( in which two or more chondrocyte are present)

Note - if no fiber is present in matrix then it is hyaline cartilage while elastic fiber look similar to hyaline but elastic fiber is present

e)bone - easiest to identify marked by presence of haversian canal system and lacuna in center having only one osteocyte.