Question

Need answers for the questions that follow, in keeping with the data provided.

LAB D - CATALYTIC HYDROLYSIS OF SALICIN DRY DRY LAB WORKSHEETS WORKSHEETS-DO NOT HAND IN Enzyme-Catalyzed Reaction: Rate law based on Figure 8: Experimental rate law: (based on experimental design) Blank Correction: 0:0GO Absorbance Readings Temperature of Water Bath #1 : 26-24 Temperature of Water Bath #2 5S 5d, Vial Time Absorbance (At) R: O 662 C: 0 002 R: 0 19 Absorbance (At) R: O 06S C: .005 R: 0 282 Time o S 구s 2775 |с: О. 222 C: 0106S25 s C:431 C: 2136s R: 0.49 : O-623 R: 0.812 R: 0.9O 4 0-332-48千 C: 0 23 2 R: 182 C: 122 : 0-410 NOERs AbsorbanceReadingand C = Corrected Absorbance Reading( eR-Bank Correction 1. Determine the rate constant for each temperature. What are the units (assuming concentration units can be used where absorbance values are used)? rale 211 DRY LAB D-CATALYTIC HYDROLYSIS OF SALICIN DRY LAB WORKSHEETS max absabanc Acid-Catalyzed Reaction: Rate law based on Figure 7: Experimental rate law: 84 (based on experimental design) Absorbance Readings Temperature of Water Bath #1: OSS Time s Absorbance (A) Tem perature of water Bath #2: 86.7-C TimeSAbsorbance (A) Vial 0 008 o 0420 : 08 3 012 6.233 6-41크 O 608 0:622 2 762 1O32 0-0-9 4 0 12S 4 89 6 S0148 8SO 218 S 2147 0-841 Absorbance of completely hydrolyzed sample (Amax): Calculated Values #2: 6 188 SS 8 Temperature of Water Bath #1 : 65 Temperature of Water Bath Vial Time In(Amax - At) Time In(Amax -A) 0183 2 401 3 30 163 696 1429 0 277 4 0 334 0:36 -0:423 |-1.46 ヲ59 7 218 2. Determine the rate constant for each temperature. What are the units? 41 DRY LAB D - CATALYTIC HYDROLYSIS OF SALIGIN DRY LAB WORKSHEETS 3. Determine the activation energy for the enzyme-catalyzed reaction. 26 31273 299.3 4. Determine the activation energy for the acid-catalyzed reaction. 5. Determine the enthalpy of reaction for the hydrolysis of salicin. 6. Sketch potential-energy diagrams: Enzyme-catalyzed reaction: Acid-catalyzed reaction Energy kJ/mol) Energy (kJ/mol) Reactants- Reactants Reaction Coordinate Reaction Coordinate Reminder: Complete the online marking module before deadine (refer to lab schedule in Course Outline) 213

Answer 1

0.5 5 Enzyme catalysed reaction at 28 C slope of the line 0.00034 0.4 е 0.3- 0.2 0 0.0 200 400600 800 1000 1200 1400 time

1.2- Enzyme catalysed reaction at 57.6°C slope of the line 0.000825 1.0 0.8 e 0.6 Co 0.2 0.0 200 400 600 800 1000 1200 1400 time

-0.20 Acid catalysed reaction at 67.5°C slope of the line -0.00013 0.25 -0.30 E-0.35 -040 0.45 0 500 1000 1500 2000 time

92-9 Acid catalysed reaction at 85.6 C slope of the line -0.00068 0.4 0.6 -0.8 1.0 1.2 1.4 500 1000 1500 2000 time

aci d catalyaed 2.4.76 KJmo) 42-78 R.

9783/mol To calcarate the E ty oP-ti meaction Weneed to use me elcttion- 凸ed reaction 8 acect coctatysed react on 9o 320 komo (Dmtǐifngane a schematic dar a a? haj been shown