Temperature can affect enzyme-catalyzed reaction rates. What is the temperature option for trypsin? What would happen if the experiment was done at trypsin’s optimum pH but at 4 degrees celsius? 50 degrees Celsius? 100 degree Celsius?
Temperature can affect enzyme-catalyzed reaction rates. What is the temperature option for trypsi...
Enzymes Temperature is an important factor that can regulate enzyme-mediated functions. Temperature is one of the tightly homeostatically controlled variables in the human body. What are the symptoms of loss of temperature control during a high fever? Explain why these symptoms occur based on enzyme function and cell signaling effects that are dependent on temperature. Based on Figure 1, explain how the temperature optimum of the activity of enzymes is expected to differ in organisms depending on where they live....
List 3 factors that affect the reaction rates of enzyme catalyzed physiological reactions.
2. List some familiar adaptations animals have to reduce the effects of temperature extremes on their many vital, enzyme-catalyzed reactions. 3. In general, the rates of chemical reactions double for every 10 degrees (Celsius) increase in temperature. What changes at the molecular level account for slower reaction rates of enzyme- catalyzed reactions at high temperatures? (Hint: enzymes are protein molecules. What happens to protein molecules when they are heated?) Why are enzyme catalyzed reactions also slower at low temperatures? (Hint:...
What will happen to a reaction that is catalyzed by a particular enzyme if the enzyme has been changed by a mutation?
What will happen to a reaction that is catalyzed by a particular enzyme if the enzyme has been changed by a mutation?
An enzyme-catalyzed reaction is carried out in a 100 mL solution containing 0.1 M TRIS buffer. Th pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H^+ were produced. What is the ratio of TRIS base to TRIS acid at the start of the experiment? What is the ration at the end of the experiment? What is the final PH?
3. "Enzymes affect reaction rates, not equilibria." This is a fundamental concept of enzyme action. Explain, in your own words, why enzymes cannot alter the equilibrium of a reaction. Then explain how enzymes can actually speed up a reaction. 4. How is the activation energy AG different from the biochemical standard free energy change AG"? 5. Explain why AG's p can be different from AG p.s. Describe the implication of this difference in terms of the direction of the reaction...
Under what circumstances does an enzyme catalyzed reaction rate resemble a non-enzyme catalyzed reaction? At very low concentrations of substrate (Km is greater than S) the Michaelis-Menton equation can be simplified to? At very high concentrations of substrate, the Michaelis-Menton equation can be simplified to? How do you determine the initial rate of reaction
An enzyme-catalyzed reaction was carried out in 5 ml of a 0.05 M phosphate buffer, pH 6.90. As a result of the reaction, 0.01 mole/liter of H+ was produced. a) What were the concentrations of each form of the buffer at the start of the reaction? b) What was the pH at the end of the reaction? c) Was this an appropriate buffer for this experiment? Why?
Lab Time Answer the Following Questions Evolution ofbubbles is usually observed when pouring H;O2 on an open wound, but not found when H2O is poured on the surface of unbroken skin. Why is this? Explain the reason for the bubbling by use of a chemical reaction. 1. 2. Estimate the optimum temperature for the catalase catalyzed reaction. What would you expect if the experiment is carried out with catalase at 50 degrees? Explain at the molecular level what change is...