Question

6. Electrophoretic mobility of a solute is given by the expression: με where q-Ze (where Z is the charge number, i.e. +1 for a cation and e 1.60x10-19 C, the charge on an electron) (Hint 1 C 1 NmW). The viscosity of water is 1.0x10-3 N sec m2 a) Calculate the mobility of bovine serum albumin (BSA) (rBSA 3.5 nm, with a charge of +6) and the mobility of the solution ions Na' and C (r0.097 nm and ra-0.181 nm) b) If you assume that the μΝα °, what is the velocity of BSA and the Ct ions when a 15,000 Volt potential is applied across a 10 cm capillary? c) In what direction will BSA and the C and Na ions travel, towards the cathode or anode and with what velocity?

Answer 1

desi X 3.lyx 3.5 9 X to = 1-451 X1。 3 х n x ,.ox10_ x 0.0 97 x1。 16 x o 2

eve cil 1 X 379ΧΟ.rel e= electric field acaass Hu- o o oo vot So Fes BsA VS

velo BSA elocty S- cu- ou - as A. Cl- and Nat-İcas veloss