which media is use to do the coagulase test?
COAGULASE TEST:
Coagulase test is used to differentiate Staphylococcus aureus (positive) from Coagulase Negative Staphylococcus (CONS).
Coagulase is an enzyme produced by S. aureus that converts (soluble) fibrinogen in plasma to (insoluble) fibrin.
STUDY QUESTIONS: STAPHYLOcocc 1. Why would you perform a coagulase test on a bacterial isolate that tested positive for mannitol salt agar fermentation? 2. Would a catalase positive test always signify the presence of Staphylococci spp.? 3. What is the medical significance of a coagulase positive test result?
MEDIA WORKSHEETxis COLOR OF MEDIA WHEN IT HAS A NEGATIVE REACTION POSITIVE REACTION COLOR OF MEDIA WHEN IT HAS A PAGE NAME OF TEST WHAT THE TEST ISREAGENTS STUDENT TEST I COMMENTS |FOUND REAGENT USED FOR -SED -MOUNTS| ON NUTRIENT GELATIN DEEPS UREA BROTH PHENYLALANINE SLANT NITRATE BROTH OXIDASE CATALASE COAGULASE BILE ESCULIN SLANT 6.5% SALT BROTH
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used to test for Oxidase? 5. Which families of organisms are differentiated by the Catalase test? What is a positive test for catalase? 6. What substrate is used to test for the presence of the Coagulase enzyme? What does Coagulase do for a bacteria that has it?
A throat culture grows normal oropharyngeal microbiota. The coagulase test for the organism is positive. What is the genus and species of the organism? 1. Staphylococcus aureus 2. Streptococcus agalactiae 3. Streptococcus pyogenes 4. Staphylococcus epidermidis
What social media do you use? Do you ever use social media for business purposes?
What social media tools do you use and why? How do you use social media to support your personal brand? Is there one social media method you prefer over another and why? Include specific examples and screen shots of your social media sites if appropriate.
RESULTS Pathogenic Potential Coagulase Production Staphylococcus aureus Wall Staphylococcus epidermidis Enterococcus faecalis 169 PARTY: Identification of Gram Positive and Gram Negative Bacteria tab Activity 82 Coagulase Test: Post-Lab
Culture site: wound White to golden, medium sized colony beta hemolytic catalase – positive coagulase latex – positive coagulase tube-negative A disk – negative CAMP test – negative Bile esculin – positive NaCl – positive Optochin disk – negative Bile solubility – negative PYR – positive ID: _____________
Culture site: stitch abscess white, medium sized colony beta hemolysis catalase – positive slide coagulase – positive tube coagulase- positive A disk – negative CAMP test – negative Bile esculin – positive NaCl – positive Optochin disk – negative Bile solubility – negative PYR – negative id the organism
Write at least three paragraph about Social Media Use. In which include What is social media? What evidence for the positive benefits of social media use? Negative of social media use?