Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes Pvu...
Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes PvuII, HincII, ClaI, and BanII, alone and in pairs. The digestion products are separated by gel electrophoresis, and the size of each fragment is determined by comparison to size standards. The fragment sizes obtained, in kilobase pairs, are given below. Draw a diagram of the 7.8-kb fragment, showing the location of the restriction sites for each enzyme.
a.Digestion with PvuII: 1.3 kb, 6.5 kb
b.Digestion with HincII: 0.5 kb, 3.3 kb, 4 kb
c.Digestion with ClaI: 1 kb, 6.8 kb
d.Digestion with BanII: 1.3 kb, 6.5 kb
e.Digestion with PvuII and ClaI: 1 kb, 5.5 kb, 1.3 kb
f.Digestion with PvuII and HincII: 0.5 kb, 0.8 kb, 2.5 kb, 4 kb
g.Digestion with HincII and ClaI: 0.5 kb, 1 kb, 3 kb, 3.3 kb
h.Digestion with HincII and BanII: 0.5 kb, 0.8 kb, 2.5 kb, 4 kb
i.Digestion with ClaI and BanII: 1 kb, 1.3 kb, 5.5 kb
Homework Answers
The
restriction site of BanII and PvuII are very close to each other so
that they gave the fragments of same size
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Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes Pvu...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
A linear piece of DNA has the following restrictions sites: You decided to set up an...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
1. A small piece of DNA was digested using restriction enzymes HindIII and PstI: A) How...
1. A small piece of DNA was digested using restriction enzymes
HindIII and PstI:
A) How many basepairs long is the fragment?
a. 900 bp
b. 2000 bp
c. 600 bp
d. 500 bp
B) How many PstI restriction sites are located within
this fragment?
a. 0
b. 3
c. 1
d. 4
e. 2
Psti Pstl Hindili 500 500 100 900
2) Consider the gel to the right. The DNA being digested is linear and 20 kb...
2) Consider the gel to the right. The DNA being digested is linear and 20 kb in length. Lane "T" is a standard DNA ladder, not a digestion. (2 points total) I Gel lanes II III IV V Which of the following is TRUE? 20 K a) The enzyme used in lane III cuts 4 times b) The enzyme used in lane II cuts 1 time c) The enzyme used in lane IV cuts 3 times d) It is likely...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments were produced of sizes 6 kb and 4 kb. The restriction enzyme HaeIII (H) also produced two fragments of sizes 9 kb and 1 kb. When the two enzymes HaeIII and EcoRI were used together, three fragments were produced of sizes 1 kb, 3 kb, and 6 kb. A 4 kb long cloned genomic probe from this region hybridized to the 3 kb and...
A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and...
A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and then with a combination of the two enzymes. The fragments obtained are: Eco RV 2 kb, 3.5 kb, 9.5 kb Bam HI 6 kb, 9 kb Eco RV and Bam H12 kb, 3.5 kb, 4 kb. 5.5 kb Draw a restriction map of the DNA fragment. (5 marks). Model of example restriction map to show you how to give your answer: Eco R1 Hind...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Lane M: a mixture of known markers increasing in size from 1 kb to 6 kb,...
Lane M: a mixture of known markers increasing in size from 1 kb
to 6 kb, in steps of 1 kb
lane 1: sample from the original plasmid DNA preparation
lane 2 : products of complete digestion with Xbal alone
lane 3: products of complete digestion with Nhel alone.
Lane 3. PruuUCIS UI compiere uiyesion WILTT III alone Lane 4: Products of complete digestion with Xbal plus Nhel M #1 #2 #3 #4 - -NW ua 중증 중증 증 증...
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
Cloning 2 Below is the restriction map of a 10 kb piece of DNA. Also shown...
Cloning 2
Below is the restriction map of a 10 kb piece of DNA. Also shown
below is a cloning vector which has two unique restriction enzyme
recognition sites, one for EcoRI (E) and one for HindIII (H). The
location of the kanamycin (kan) and ampicillin (amp) resistance
genes is also shown. Kanamycin and ampicillin are antibiotics that
are commonly used to select transformed E. colicells (consult the
Lab Manual for more information). Note that the HindIII site is
located...
A linear piece of DNA has the following restrictions sites: Xbal Noti Psti EcoRi 2 kb 4 kb -> 1 kb 2 kb 3 kb You decided to set up an experiment where you added one of these restriction enzymes to this DNA. After this DNA was digested with that restriction enzyme, you separated the resulting fragment(s) using agarose electrophoresis. After the gel was stained with ethidium bromide, you observed the following gel (the DNA ladder is your reference standard...
A linear piece of DNA has the following restrictions sites:
You decided to set up an experiment where you added one of these
restriction enzymes to this DNA. After this DNA was digested with
that restriction enzyme, you separated the resulting fragment(s)
using agarose electrophoresis. After the gel was stained with
ethidium bromide, you observed the following gel (the DNA ladder is
your reference standard and is comprised of a series of DNA
fragments of known length).
a) Which restriction...
1. A small piece of DNA was digested using restriction enzymes
HindIII and PstI:
A) How many basepairs long is the fragment?
a. 900 bp
b. 2000 bp
c. 600 bp
d. 500 bp
B) How many PstI restriction sites are located within
this fragment?
a. 0
b. 3
c. 1
d. 4
e. 2
Psti Pstl Hindili 500 500 100 900
2) Consider the gel to the right. The DNA being digested is linear and 20 kb in length. Lane "T" is a standard DNA ladder, not a digestion. (2 points total) I Gel lanes II III IV V Which of the following is TRUE? 20 K a) The enzyme used in lane III cuts 4 times b) The enzyme used in lane II cuts 1 time c) The enzyme used in lane IV cuts 3 times d) It is likely...
A linear fragment of DNA is cleaved with the individual restriction enzymes Hindill and Smal, and then with a combination of the two enzymes. The fragments obtained are: Eco RV 2 kb, 3.5 kb, 9.5 kb Bam HI 6 kb, 9 kb Eco RV and Bam H12 kb, 3.5 kb, 4 kb. 5.5 kb Draw a restriction map of the DNA fragment. (5 marks). Model of example restriction map to show you how to give your answer: Eco R1 Hind...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Lane M: a mixture of known markers increasing in size from 1 kb
to 6 kb, in steps of 1 kb
lane 1: sample from the original plasmid DNA preparation
lane 2 : products of complete digestion with Xbal alone
lane 3: products of complete digestion with Nhel alone.
Lane 3. PruuUCIS UI compiere uiyesion WILTT III alone Lane 4: Products of complete digestion with Xbal plus Nhel M #1 #2 #3 #4 - -NW ua 중증 중증 증 증...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
Cloning 2
Below is the restriction map of a 10 kb piece of DNA. Also shown
below is a cloning vector which has two unique restriction enzyme
recognition sites, one for EcoRI (E) and one for HindIII (H). The
location of the kanamycin (kan) and ampicillin (amp) resistance
genes is also shown. Kanamycin and ampicillin are antibiotics that
are commonly used to select transformed E. colicells (consult the
Lab Manual for more information). Note that the HindIII site is
located...
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