Homework Answers
ANS; 3 the nonspecific dye when bind to the double stranded DNA it florescence more on the other hand in the presence of single stranded DNA it is not florescence.
Example; the detection of double stranded DNA by using syber green , this dye bind to the minor groove of the double stranded DNA and increase the florescence but in the presence of single stranded give very light florescence.
ans; 4 the melting curve in the QPCR tell us about the specificity of the products. these analysis check that the primer we have to used in the reaction give a specific amplified products or no specific products.if the result show more then one peak it means the product is non specific.
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3. How does the fluorescence of nonspecifc reporter dyes differ in the presence of single-strande...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
Telomere Length Estimation Objective To estimate the length of telomeres on your extracted gDNA. Background Telomeres...
Telomere Length Estimation Objective To estimate the length of telomeres on your extracted gDNA. Background Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
61 Analyze and comment on the "You're The Economist" box on page 140. How does the...
61 Analyze and comment on the "You're The Economist" box on page 140. How does the elasticity of demand for tobacco products differ for children versus adults? How can government use this difference? Please post your main answer and two comments on two different days. YOU'RE THE ECONOMIST Applicable Concepts: Tot Marginal Utility Why Is Water Less Expens Adam Smith posed a paradox in The Wealth of Nat Water is essential to life and therefore to life and therefore should...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
What genes (or kind of genes) will you focus on in your investigative research? Provide 2-3...
What genes (or kind of genes) will you focus on in your investigative research? Provide 2-3 reasons for your choice of these gene/s. Starting with the bone sample itself, what methods will you use to get DNA that you can sequence? Which “ingredients” in your lab reactions will determine which gene or genes are copied? How is it that these ingredients are able to target a specific gene? What method will you use to see if your efforts to copy...
bio lab palentology questions that is the entire lab i did not leave anything out ~...
bio lab palentology questions
that is the entire lab i did not leave anything out ~
Lab 11: The Witte il An Introduction to Paleontology 1. Paleontology at the Witte: Paleontology is the study of fossils. Traditionally, multiple subdisciplines can be defined. For example, Paleobotany is the study of fossil plants, Paleodimatology is the study of ancient climates, and Palynology is the study of fossil pollen and spores. Paleontology differs from Archaeologyas the former includes the study of modern humans....
please help me solve this ANAL 364-Qualitatitive Analysis Discussion Topics b) You did not wash the...
please help me solve this
ANAL 364-Qualitatitive Analysis Discussion Topics b) You did not wash the filter paper with hot water before filtering in the step used to separate PbCl2 from AgCLand Hg.Cl 5. AIPO4, with a Kg of 9.8 x 1022 is less soluble than Cas(PO4)2, which has a Kp of 2.0 x 1029 Why? 6. Would the solubility of AlIPO, with a Kp of 9.8 x 102 or Cas(POa), which has a Kp of 2.0x 10-9 be affected...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
61 Analyze and comment on the "You're The Economist" box on page 140. How does the elasticity of demand for tobacco products differ for children versus adults? How can government use this difference? Please post your main answer and two comments on two different days. YOU'RE THE ECONOMIST Applicable Concepts: Tot Marginal Utility Why Is Water Less Expens Adam Smith posed a paradox in The Wealth of Nat Water is essential to life and therefore to life and therefore should...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
bio lab palentology questions
that is the entire lab i did not leave anything out ~
Lab 11: The Witte il An Introduction to Paleontology 1. Paleontology at the Witte: Paleontology is the study of fossils. Traditionally, multiple subdisciplines can be defined. For example, Paleobotany is the study of fossil plants, Paleodimatology is the study of ancient climates, and Palynology is the study of fossil pollen and spores. Paleontology differs from Archaeologyas the former includes the study of modern humans....
please help me solve this
ANAL 364-Qualitatitive Analysis Discussion Topics b) You did not wash the filter paper with hot water before filtering in the step used to separate PbCl2 from AgCLand Hg.Cl 5. AIPO4, with a Kg of 9.8 x 1022 is less soluble than Cas(PO4)2, which has a Kp of 2.0 x 1029 Why? 6. Would the solubility of AlIPO, with a Kp of 9.8 x 102 or Cas(POa), which has a Kp of 2.0x 10-9 be affected...
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