The answers are B and D respectively, but I am stuck on how to solve these types of questions. Any help would be appreciated. Thank you!!
Homework Answers
![lin e wecwe Burk plot. Trn [s] Vmtx. V is the reacHon veloci 1km the Mitae-Menten conslant ne. sente df inhibifov line weaver](http://img.homeworklib.com/images/247caea1-750b-4d8c-9329-d529a8262137.png?x-oss-process=image/resize,w_560)
Add Answer to:
The answers are B and D respectively, but I am stuck on how to solve these types of questions. An...
Please help me with these two problems. I will rate the answers. 1. The HIV-1 protease,...
Please help me with these two problems. I will rate the
answers.
1. The HIV-1 protease, an important enzyme in the life cycle of the human immunodeficiency virus-1 (HIV-1), is a good drug target for the treatment of HIV and AIDS. A protein produced by the virus, p6*, is an HIV-1 protease inhibitor. The activity of the HIV-1 protease was measured in the presence and absence of p6* using an assay involving an artificial substrate, as shown below. NH Lys...
Can help me with these questions please. 30. Sphingosine-1-phosphate (S1P) is important for cell survival. The...
Can help me with these questions please. 30. Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below. Sphingosine (uM) vo (mg /min) vo (mg /min) with inhibitor 2.5 32.3 8.5 3.5 40 11.5 5.0 50.8 14.6 10 72...
need B C and D done please please please help!!! 1. You measure the initial rate...
need B C and D done please please please help!!!
1. You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data was obtained: V. (-) Inhibitor (mm/min) (+) Inhibitor (mM/min) 17 [S] (MM) 0.0001 0.0002 0.0005 0.001 0.002 Please submit calculations and graph for full credit! Note: You are required to use Excel to generate the Lineweaver-Burk plot (a) (10 points) Create a Lineweaver-Burk...
30. Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP...
30. Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below. Sphingosine (UM)v. (mg/min) v. (mg/min) with inhibitor 2.5 32.3 8.5 3.5 40 11.5 50.8 14.6 25.4 87.7 43.9 115.4 70.8 Construct a Lineweaver-Burk plot to answer the following...
Can anyone help with parts D and E? Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase...
Can anyone help with parts D and E?
Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
show work please! with inhibitor (umoles/min/mg) [S] (mm) v- no inhibitor (umoles/min/mg) V - with inhibitor...
show work please!
with inhibitor (umoles/min/mg) [S] (mm) v- no inhibitor (umoles/min/mg) V - with inhibitor (umoles 3.0 2.29 x 103 1.83 x 103 5.0 or ble 3.20 x 103 Boristeder s obre o 2.56 x 103 7.0 3.86 x 103 USD 3.09 x 103 0 9. 4 .36 x 100W 3.49 x 103 0 11. 4 .75 x 108 3.80 x 103 Draw Lineweaver-Burk plots for the enzyme data shown above. When present, I = 0.200 m a) What...
5.5 5 4.5 حشر ان (%/D) [A]/1 نن ان • No inhibitor FPH 60mg/ml AFPA 90mg/ml...
5.5 5 4.5 حشر ان (%/D) [A]/1 نن ان • No inhibitor FPH 60mg/ml AFPA 90mg/ml دہ НАН 0 -30 -20 -10 0 10 20 1/[S] (mL/mg) 30 40 50 What is the approximate Km for the enzyme in the graph above, to one significant figure, without any inhibitor? mg/ml Figure 10. Activity v metal ions regression analysis graphs for rainbow trout GST in the presence of 5 different (a) (Ag)(b)( (d) Mg concentrations. c) Cr" • Control 30.01 M...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor 0.02 0.04 0.10 0.25 1.00 2.50 0.8 2.9 8.6 24 36 50 Plot the data for the kinetics of the enzyme (with and without the inhibitor) in a double reciprocal (Lineweaver-Burk) plot. Keep in mind that the x axis is 1/[S] and the y axis is 1/Vo. If you are using Excel you want to choose the (x,y) scatter plot (without a ne). You...
Prostaglandins are a class of eicosanoids (fatty acid derivatives) with a variety of extremely potent actions...
Prostaglandins are a class of eicosanoids (fatty acid derivatives) with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid, arachidonic acid, in a reaction catalyzed by the enzyme, prostaglandin endoperoxide synthase. This uses oxygen to convert arachidonic acid to PGG2 (a prostaglandin). Ibuprofen inhibits this enzyme and is a fever reducer and anti-inflammatory agent. The kinetic data is given below...
LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the...
LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the final reading on your blood glucose meter for one of your samples is 385 mg/dL. You need your data to be in units of mm (millimolar, which is millimoles per liter, abbreviated as mmol/L). Show, in a step-by-step conversion, how you would convert 385 mg/dL to the proper answer in mM. 385 mg dlº= ?mm immoles 2. Assuming that the reaction occurred over a...
Please help me with these two problems. I will rate the
answers.
1. The HIV-1 protease, an important enzyme in the life cycle of the human immunodeficiency virus-1 (HIV-1), is a good drug target for the treatment of HIV and AIDS. A protein produced by the virus, p6*, is an HIV-1 protease inhibitor. The activity of the HIV-1 protease was measured in the presence and absence of p6* using an assay involving an artificial substrate, as shown below. NH Lys...
need B C and D done please please please help!!!
1. You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data was obtained: V. (-) Inhibitor (mm/min) (+) Inhibitor (mM/min) 17 [S] (MM) 0.0001 0.0002 0.0005 0.001 0.002 Please submit calculations and graph for full credit! Note: You are required to use Excel to generate the Lineweaver-Burk plot (a) (10 points) Create a Lineweaver-Burk...
30. Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below. Sphingosine (UM)v. (mg/min) v. (mg/min) with inhibitor 2.5 32.3 8.5 3.5 40 11.5 50.8 14.6 25.4 87.7 43.9 115.4 70.8 Construct a Lineweaver-Burk plot to answer the following...
Can anyone help with parts D and E?
Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
show work please!
with inhibitor (umoles/min/mg) [S] (mm) v- no inhibitor (umoles/min/mg) V - with inhibitor (umoles 3.0 2.29 x 103 1.83 x 103 5.0 or ble 3.20 x 103 Boristeder s obre o 2.56 x 103 7.0 3.86 x 103 USD 3.09 x 103 0 9. 4 .36 x 100W 3.49 x 103 0 11. 4 .75 x 108 3.80 x 103 Draw Lineweaver-Burk plots for the enzyme data shown above. When present, I = 0.200 m a) What...
5.5 5 4.5 حشر ان (%/D) [A]/1 نن ان • No inhibitor FPH 60mg/ml AFPA 90mg/ml دہ НАН 0 -30 -20 -10 0 10 20 1/[S] (mL/mg) 30 40 50 What is the approximate Km for the enzyme in the graph above, to one significant figure, without any inhibitor? mg/ml Figure 10. Activity v metal ions regression analysis graphs for rainbow trout GST in the presence of 5 different (a) (Ag)(b)( (d) Mg concentrations. c) Cr" • Control 30.01 M...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor 0.02 0.04 0.10 0.25 1.00 2.50 0.8 2.9 8.6 24 36 50 Plot the data for the kinetics of the enzyme (with and without the inhibitor) in a double reciprocal (Lineweaver-Burk) plot. Keep in mind that the x axis is 1/[S] and the y axis is 1/Vo. If you are using Excel you want to choose the (x,y) scatter plot (without a ne). You...
Prostaglandins are a class of eicosanoids (fatty acid derivatives) with a variety of extremely potent actions on vertebrate tissues. They are responsible for producing fever and inflammation and its associated pain. Prostaglandins are derived from the 20-carbon fatty acid, arachidonic acid, in a reaction catalyzed by the enzyme, prostaglandin endoperoxide synthase. This uses oxygen to convert arachidonic acid to PGG2 (a prostaglandin). Ibuprofen inhibits this enzyme and is a fever reducer and anti-inflammatory agent. The kinetic data is given below...
LINEWEAVER-BURK plot 1. You perform the hydrolysis reaction as required in the lab experiment, and the final reading on your blood glucose meter for one of your samples is 385 mg/dL. You need your data to be in units of mm (millimolar, which is millimoles per liter, abbreviated as mmol/L). Show, in a step-by-step conversion, how you would convert 385 mg/dL to the proper answer in mM. 385 mg dlº= ?mm immoles 2. Assuming that the reaction occurred over a...
Most questions answered within 3 hours.
-
How much energy is required to vaporize 175 g of butane at its
boiling point? The...
asked 7 minutes ago -
X has a normal distribution with the given mean and
standard deviation. Find the indicated probability....
asked 9 minutes ago -
Calculate the probability that a particle will be found in a
tiny slice of space between...
asked 12 minutes ago -
Developed countries with some of the highest GDPs per
capita:
a)are not found outside of Europe...
asked 14 minutes ago -
draw a diagram illustrating the three ways of genetic
information flow
asked 30 minutes ago -
CASE 3-1 YOU CAN’T GET THERE FROM HERE: UBER SLOW ON
DIVERSITY
Established in 2009, Uber...
asked 30 minutes ago -
A local AM radio station broadcasts at an energy of
4.39×10-31 kJ/photon.
Calculate the frequency at...
asked 53 minutes ago -
Smithson Cutting is opening a new line of scissors for
supermarket distribution. It estimates its fixed...
asked 58 minutes ago -
A student working in a genetics lab was given the task of
studying a new Drosophila...
asked 59 minutes ago -
Object A is moving due east, while object B is moving due north.
They collide and...
asked 1 hour ago -
Use the de Broglie relationship (l = h/mu where u is speed) to
determine the wavelengths...
asked 1 hour ago -
An elliptical hair that is 80µ by 120µ in minor and
major diameters that is 10...
asked 1 hour ago