Question

5. Below are the absorption spectra of solutions of various concentrations of BSA with a new protein assay reagent. [BSA]-0 g/d [BSA] 4.95 x 10-3 g/dl, BSA] 9.80 x 103 g/dl, [BSA] 14.56 x 10-3 g/dl, and [BSA]-19.23 x 10-3 g/d. (a) (0.5 point) Which wavelength would be most useful in order to construct a standard curve? 2u (b) (0.5 point) Would this be a good protein assay to determine BSA concentration that is above 20.00 x 10-3 05 gd1? 00800 Wavelength (nm) 300 400 1000

Answer 1

(a) 650 nm would be better option as we see steady increase in absorption at that wavelength whereas for 450 nm case after a decrease it has become saturated which makes it difficult to dewa the standard curve.

(b) No, as we can clearly see the increase in absorbance at 650 nm is getting saturated as the relative changes between consecutive data points are decreasing.So, above 20 ×10^-3 g/dL , the linearity of standard curve will not be retained.So, it cannot be used.