3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when in...
3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when introduced to the plasma membrane after trafficking?
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3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when in...
If you deleted the ER N-terminal signal sequence from a gene that encodes a protein, where...
If you deleted the ER N-terminal signal sequence from a gene that encodes a protein, where would the protein end up after synthesis? (Assume no other signal sequences are present) The golgi The smooth ER The protein would be secreted from the cell The cytosol The lysosome
1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal)...
1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal) 2. Name 4 organelles that require organelle specific targeting sequences in polypeptide 3. Name 3 final locations for proteins with ER signal sequence 4. Satranslational translocation: (a) Name the protein that the signal sequence binds to (b) Name the protein SRP binds to 5. State the function of signal peptidase 6. Name a membrane protein that is not a single pass membrane protein 7....
1. (14pts) A. What are the functions of the signal recognition particle (SRP) and translocon in p...
PLEASE ANSWER ALL THE QUESTIONS IN EACH PART. THANK YOU!
1. (14pts) A. What are the functions of the signal recognition particle (SRP) and translocon in protein transport to the ER? SRP Translocon B. How does the translocon determine which orientation to insert membrane proteins? C. How do budding & fusion affect the orientation of integral membrane proteins during vesicle transport? D. Consider a ligand-gated ion channel at the plasma membrane that binds to ligands in the cytoplasm. From a...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
3. You modify GDP (green fluorescent protein) to contain a signal sequence you believe is a...
3. You modify GDP (green fluorescent protein) to contain a signal sequence you believe is a novel ER import signal at the N-terminus and a stop transfer sequence 43 amino acids from the N terminal import signal. When this protein is expressed in cells, you see 70% GFP in the cytoplasm and the remainder in the ER. You assume GFP import is occurring. However you cannot rule out some GFP might not fold properly even though it is fluorescent, perhaps...
A protein has the following N-terminal sequence and the following organization of signal/stop/start sequences. Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-… i)...
A protein has the following
N-terminal sequence and the following organization of
signal/stop/start sequences.
Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-…
i) What is the topology of this protein in the cell membrane?
The red segments correspond to either signal sequences or stop
transfer sequences. The orange segments correspond to start
transfer sequences
ii) What is the N-terminal sequence of the protein, after
processing
iii) Is this a eukaryotic or prokaryotic protein? Why?
Signal Stop Start Stop Start
35. Insulin is a peptide (protein) hormone secreted by B-cells located in the pancreas. To be...
35. Insulin is a peptide (protein) hormone secreted by B-cells located in the pancreas. To be protein) hormone used to regulate the level of glucose in the bloodstream. It is produced and performed. cated in the pancreas. To better understand how insulin is secreted, the following experiments were a. The mature me mature mRNA for insulin encodes a protein that is 110 amino acids in length. If the genes t is 110 amino acids in length. If the gene is...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
n 13 You have found a mutant that has a G-protein that can only bind GDP...
n 13 You have found a mutant that has a G-protein that can only bind GDP and cannot bind GTP. Which of the following might you expect in the mutant cells? red ed out of ag question Select one: a. The secondary messenger would be made constantly. b. The cells would have more energy availability due to higher GTP binding. c. The secondary messenger would not be made. O d. The primary messenger would not be able to bind to...
23. Consider these three sequences contained within single beta strands. Which is most likely to have ionic interaction...
23. Consider these three sequences contained within single beta strands. Which is most likely to have ionic interactions between side chains at physiological pH? Explain your choice. Sequence 1: Ala-Glu- Leu- Arg Sequence 2: Ala - Glu - Arg-Leu Sequence 3: Leu - Ala - Glu- Arg 24. The equilibrium between the native state (N) and the unfolded state (U) of a protein can be represented by the chemical equation N U, with a AG value that represents the equilibrium....
1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal) 2. Name 4 organelles that require organelle specific targeting sequences in polypeptide 3. Name 3 final locations for proteins with ER signal sequence 4. Satranslational translocation: (a) Name the protein that the signal sequence binds to (b) Name the protein SRP binds to 5. State the function of signal peptidase 6. Name a membrane protein that is not a single pass membrane protein 7....
PLEASE ANSWER ALL THE QUESTIONS IN EACH PART. THANK YOU!
1. (14pts) A. What are the functions of the signal recognition particle (SRP) and translocon in protein transport to the ER? SRP Translocon B. How does the translocon determine which orientation to insert membrane proteins? C. How do budding & fusion affect the orientation of integral membrane proteins during vesicle transport? D. Consider a ligand-gated ion channel at the plasma membrane that binds to ligands in the cytoplasm. From a...
5. (9pts) You are studying an ER membrane protein, YFG1 (outlined in yellow), with three transmembrane domains (rectangles A, B & C at the amino acid positions according to the following map, connected by soluble domains (wavy lines): N-term 10 10 Com Amino acid position (not to scale) 30 60 80 140 160 310 ABCI YFG1 alkaline phosphatase To determine how the protein is arranged in the ER membrane, you construct a series of shortened YFG1 proteins that are connected...
A protein has the following
N-terminal sequence and the following organization of
signal/stop/start sequences.
Met-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser-Arg-…
i) What is the topology of this protein in the cell membrane?
The red segments correspond to either signal sequences or stop
transfer sequences. The orange segments correspond to start
transfer sequences
ii) What is the N-terminal sequence of the protein, after
processing
iii) Is this a eukaryotic or prokaryotic protein? Why?
Signal Stop Start Stop Start
35. Insulin is a peptide (protein) hormone secreted by B-cells located in the pancreas. To be protein) hormone used to regulate the level of glucose in the bloodstream. It is produced and performed. cated in the pancreas. To better understand how insulin is secreted, the following experiments were a. The mature me mature mRNA for insulin encodes a protein that is 110 amino acids in length. If the genes t is 110 amino acids in length. If the gene is...
n 13 You have found a mutant that has a G-protein that can only bind GDP and cannot bind GTP. Which of the following might you expect in the mutant cells? red ed out of ag question Select one: a. The secondary messenger would be made constantly. b. The cells would have more energy availability due to higher GTP binding. c. The secondary messenger would not be made. O d. The primary messenger would not be able to bind to...
23. Consider these three sequences contained within single beta strands. Which is most likely to have ionic interactions between side chains at physiological pH? Explain your choice. Sequence 1: Ala-Glu- Leu- Arg Sequence 2: Ala - Glu - Arg-Leu Sequence 3: Leu - Ala - Glu- Arg 24. The equilibrium between the native state (N) and the unfolded state (U) of a protein can be represented by the chemical equation N U, with a AG value that represents the equilibrium....
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