Question

8.   Can you use this method (restriction mapping using restriction enzymes) to determine precise fragment lengths? Explain.

9.   In this experiment double digests were performed on the complete digests of the single enzymes. What would the original gel look like if the Dra I fragments were isolated and digested separately with KspAI?

(Gel Key: K=KspAI; D=Dra I; B=both; X=control)

Gel D-3 DNA only Ladder both K 1.30cm(supercol ling) Dem percoilin 1.65em 2.7om 2.25cm 2.90cm 4.95cm 6.75m 7.25cm 7.20cm 8.50am 9.25cm 9.30cm

Answer 1

8. Yes, restriction digestion gives the perfect fragment length of each restriction enzymes used. Also it can used to map the restriction enzyme present in the DNA. If doing double digest we can able to make the accurate size of the fragments.

9.

CA. 96Cm AT