1. Calculate KM and Vmax from the following data: vo (mM S- 0.34 0.53 0.74 0.91 1.04 [S] (μΜ) 0.1 0.2 0.4 0.8 1.6 1. Calculate KM and Vmax from the following data: vo (mM S- 0.34 0.53 0.74 0....
11. Calculate KM and Vmax from the following data: [S] (UM), (mm/s) 0.1 0.34 0.53 0.4 0.74 0.8 0.91 1.04 0.2
The following data set was collected from an experiment conducted in the lab where a new enzyme is being characterized. Use the Lineweaver-Burk method in order to determine the values of KM and Vmax. Complete your work on a separate piece of paper and upload the excel file or picture of your work. Your work must include the following: Table of reciprocals Reciprocal plot (straight-line graph) Calculations and results for KM and Vmax In order to get full credit for...
Can help me with these questions please. 30. Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below. Sphingosine (uM) vo (mg /min) vo (mg /min) with inhibitor 2.5 32.3 8.5 3.5 40 11.5 5.0 50.8 14.6 10 72...
The value of Km for the shown data for a hexokinase-catalyzed reaction is with the unit of . The value of Vmax for. the same reaction is with the unit of . Be sure to give the values with the correct number of significant figures. You might have to construct a kinetic plot. For units, choose one answer from (uM, 1/ UM, HM/second, uM x second, mM, 1/mM, second, 1/second, mM/second, mM x second) vo (mM/sec) Glucose concentration (mm) 0.10...
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1) 0.1 3.33 0.2 5.00 0.5 7.14 0.8 8.0 1.0 8.33 2.0 9.09 a.) From a double-reciprocal plot of the data, determine Km and Vmax. b.) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number.
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
-1,2,3,4,5,63 and transition matrix Consider a discrete time Markov chain with state space S 0.8 0 0 0.2 0 0 0 0.5 00 0.50 0 0 0.3 0.4 0.2 0.1 0.1 0 0 0.9 0 0 0 0.2 0 0 0.8 0 0.1 0 0.4 0 0 0.5 (a) Draw the transition probability graph associated to this Markov chain. (b) It is known that 1 is a recurrent state. Identify all other recurrent states. (c) How many recurrence classes are...
Determine the km and vmax from this data. 1/[S] 0.1 0.033333333 0.02 0.01 0.006666667 0.002 1/V0 34.3 31.95 27.2 25.51 20.16 19.6
1. an enzyme is observed to approach an initial velocity of 0.1M/s as more and more [S] is added to the reaction. additionally when V0 is equal to 0.05 M/s [S] is equal to 0.6 M. based on this information what is Vmax and Km? 2. If a psrticular enzyme has a Vmax of 0.4 M/s, and a Km of 20 mM, what is V0 wjen [S] = 0.8 M? 3. We have discussed which amino acids chymotrypsin cleaves at,...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....