Determine the km and vmax from this data. 1/[S] 0.1 0.033333333 0.02 0.01 0.006666667 0.002 1/V0...

Question

Question

Determine the km and vmax from this data.

Answer 1

Answer #1

1/V0 = (Km/Vm[S])+1/Vm

y = mx+c

comparing from the chat value of slope and intercept

m = 136.18 = Km/Vm

c = 22.56 = 1/Vm

so Vm = 0.044

and Km = 6

Answer 2

Similar Homework Help Questions

1. Calculate KM and Vmax from the following data: vo (mM S- 0.34 0.53 0.74 0.91 1.04 [S] (μΜ) 0.1 0.2 0.4 0.8 1.6 1. Calculate KM and Vmax from the following data: vo (mM S- 0.34 0.53 0.74 0....

1. Calculate KM and Vmax from the following data: vo (mM S- 0.34 0.53 0.74 0.91 1.04 [S] (μΜ) 0.1 0.2 0.4 0.8 1.6 1. Calculate KM and Vmax from the following data: vo (mM S- 0.34 0.53 0.74 0.91 1.04 [S] (μΜ) 0.1 0.2 0.4 0.8 1.6
11. Calculate KM and Vmax from the following data: [S] (UM), (mm/s) 0.1 0.34 0.53 0.4...

11. Calculate KM and Vmax from the following data: [S] (UM), (mm/s) 0.1 0.34 0.53 0.4 0.74 0.8 0.91 1.04 0.2
1. Show, using the Michaelis-Menten equation, that when [S] >>> Km, vo = Vmax. Show, using...

1. Show, using the Michaelis-Menten equation, that when [S] >>> Km, vo = Vmax. Show, using the M-M equation that when [S] <<<Km, vo =[S][Et]kcat/Km. 2. What is Vmax? Provide both a mathematical and written description of Vmax? How can Vmax be experimentally altered? How can we use Vmax to determine the turnover number (kcat) of an enzyme-catalyzed reaction? What is the major challenge of determining Vmax from an Michaelis-Menten plot?

1. Define Vmax and Km (Michaelis constant). What is the mathematical relationship between these two values?...

1. Define Vmax and Km (Michaelis constant). What is the mathematical relationship between these two values? 2.What does Km tell you about the affinity of an enzyme for its substrate? 3.Why do we use the slope between the first two data points of Absorbance vs Time to determine V0 when studying enzyme kinetics? Why not use the slope of the entire line?
Kcat =30.0 s-1 Km= 0.005 M Operating at 1/4 Vmax What is [S] ? The solutions...

Kcat =30.0 s-1 Km= 0.005 M Operating at 1/4 Vmax What is [S] ? The solutions manuel doesn't explain the problem well. Whete does the 0.33 km come from? For part 2 : Plug in [S]= 1/2 Km, 2 Km, and 10 Km Where does the 1.5 Km come from? Answer (a) Here we want to find the value of [S] when Vo = 0.25 V max. The Michaelis-Menten equation is V = Vmax[S]/(Km + [SD so V = Vmax...
a. what are the values of Vmax and Km in the abscence if the inhibitor what...

a. what are the values of Vmax and Km in the abscence if the inhibitor what are the values of Vmax and Km in the presence of the inhibitor? b. what type of inhibition is it? c. what is the dissociation constant (Ki) of the inhibition? ***d. graph a linear scatter plot including equation. Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...

Given the following data (generated from a series of assays), what is the Km and Vmax...

Given the following data (generated from a series of assays), what is the Km and Vmax for this enzyme? Include units and be careful of significant figures. [S], μM V(μM/min) 10.0 32.4 25.0 66.3 50.0 102 150.0 160 300.0 185
Interpret the data above 1. What is the aporoximate Km and Vmax (units matter) 2.Briefly explain...

Interpret the data above 1. What is the aporoximate Km and Vmax (units matter) 2.Briefly explain how you found each 3.if you used 0.020 microM enzyme in your studies, what is kcat in units of s^-1? Show your work units matter 4. Does this enzyme appear to display cooperativity? Explain how you came up to that conclusion. Directions: Below are data from 4 separate experiments that you must analyze/evaluate. Using the information from all 4 experiments, you must then propose...
how did they get these answers? we Vulins Hemoglobin/Am 1. An allosteric interaction between a) binding of a molecul...

how did they get these answers? we Vulins Hemoglobin/Am 1. An allosteric interaction between a) binding of a molecule to a bir 0 You measure the initial rate of an enzyme reaction as a function of substrate concentration in the presence and absence of an inhibitor. The following data are obtained: [S] Vo -Inhibitor +Inhibitor 17 29 0.0001 0.0002 0.0005 0.001 0.002 0.005 0.01 0.02 0.05 0.1 100 100 100 98 99 100 100 0.2 a) What is the Vmax...

How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept)...

How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept) O km = (-1) * (x-intercept) O km = 1/(x-intercept) 0 Km = s;lope