How would you design an experiment to test whether a tranduction event occured between two different bacterial strains?
U tube experiment can be performed for testing transduction between bacterial strain.
U tube is designed to place filter between the two sides of the tube. On either side are two auxotrophic bacterial strains separated by a filter through which only viral particles can pass. Transduction event is mediated by a phage particles. Phage particles infect a host bacterium and insert its genome into bacterial genome. During packing process phage genome is excised along with bacterial genes and packaged into phage particles. If this phage particles infects other bacterial strain, the genes form previous strain are transferred to this bacterial strain. Bacterial strains are auxotrophic for one or more nutrients, therefore if transduction has taken place they become prototrophs and grown in medium lacking the nutrients for which they are auxotrophs.
bacterial strain A: auxotroph for histidine, prototroph for leucine
bacterial strain B: auxotroph for leucine, prototroph for histidine.
if transduction has taken place from A to B , bacterial strain B will receive the leucine biosynthesis gene and grow in medium lacking leucine as opposed to previously
How would you design an experiment to test whether a tranduction event occured between two different bacterial strains?
Consider that the zebrafish microbiome has more than just two bacterial strains within it. How do you think adding a third (or fourth, fifth, etc.) bacterial member into a competition would potentially affect the results you gathered from this experiment?
(5) (a) Describe how you would design a manipulative experiment to demonstrate that longer manes in lions are selected for by female choice. (b) What would be the null hypothesis? (6) Two species of insects (the Kamarla beetle and the Chelsea beetle) are both bright orange in color and are both unpalatable to birds. Describe an experimental design to test whether or not they are mutualists.
original strains:
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene in the Hfr strain. Be sure to label distances between markers. You have two strains of...
If you were going to design an experiment on the effects of air pollution, how would you account for the effects that variations in health, gender, age, and ethnicity can cause? If you were going to measure whether two groups differ in a genetic predisposition to heart disease, stroke, or hypertension, how would you make sure that difference in air pollution levels experienced by the two groups didn't confound your results?
You are studying a virus that infects mammals. You are specifically interested in whether the virus frequently jumps from one mammal species to another or whether different strains remain specific to certain hosts over evolutionary time. How could you use phylogenetic methods to test this?
2. Design an experiment to test whether the attack of the nucleophile in an SN2 reaction is frontside attack or backside attack. Describe with words and reactions.
Design an experiment that would test the effect of removing heat energy from a slightly stretched rubber band. You will not perform this experiment, but the materials you intend to use should be ones you would normally find at a college. Include in your design: A list of the materials required. A brief description of the procedure you would use (a diagram might be useful). The observations you would expect to see. Explain these observations in terms of both free...
25. A cross is made between two different true-breeding strains of daylilies, both of which have white flowers. All of the F1 generation plants have yellow flowers. When an F, offspring is crossed with either one of the parental strains, half of the offspring have yellow flowers and half of them have white flowers. Propose an explanation for this outcome. In your answer, you should describe the genotypes of the two parental strains and the F1 offspring, and also explain...
Suppose you cross two inbred (purebred) strains of mice, black and white, that have different average diastolic arterial blood pressure values, 99 mm Hg (black) and 141 mm Hg (white). You obtain F1’s with an intermediate average value of 120 (ranging between 116 and 124). After interbreeding the F1’s to produce F2’s, you measure the diastolic arterial blood pressure of these F2’s. You find that only 1 out of 1100 mice shows a blood pressure like the original parental black...
Wild harebells have blue flowers. Two strains of harebells carry recessive alleles that give the plants white flowers. a. Describe a cross to determine whether the white-flower causing alleles in these two strains are variants of the same gene or different genes, and the progeny phenotypes you expect to find in each case. b. You results from the cross above indicate that the two strains carry alleles at different genes. What phenotypic ratio do you expect to find among the...