Question

Help me to describe what is happening in the results of affinity chromatography of con A experiment , based on this elution graph. Thank you

Answer 1

Affinity chromatography is a method of separating biochemical mixture based on a highly specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid. It is a type of chromatographic laboratory technique used for purifying biological molecules within a mixture by exploiting molecular properties. Organic macromolecules, for example, catalysts and different proteins, communicate with different atoms with high specificity through a few unique sorts of securities and association. Such collaborations including hydrogen holding, ionic connection, disulfide spans, hydrophobic association, and the sky is the limit from there. The high selectivity of fondness chromatography is caused by enabling the coveted particle to interface with the stationary stage and be bound inside the section with the end goal to be isolated from the undesired material which won't connect and elute first.The molecules no longer needed are first washed away with a buffer while the desired proteins are let go in the presence of the eluting solvent (of higher salt concentration). This process creates a competitive interaction between the desired protein and the immobilized stationary molecules, which eventually lets the now highly purified proteins be released.Affinity chromatography can be used to purify and concentrate a substance from a mixture into a buffering solution, reduce the amount of unwanted substances in a mixture, identify the biological compounds binding to a particular substance, purify and concentrate an enzyme solution.