Question

affinity chromatography is often used as a technique to purify cell-surface receptors. describe the steps you would take to purift rhe cell-surface receptor using this technique

Answer 1

Affinity chromatography separates molecules on the basis of their interaction with other molecules. This is based upon affinity between ligand-receptor, enzyme-substrate, antigen-antibody etc.

If we want to purify cell surface receptor than -

1. Prepare a column for affinity chromatography. This is stationary phase.

2. Add the mixture containing receptor to the column.

3. Let it run.

The column contains ligand molecules which are complementary in shape and size of the receptor we want to separate. So, during run, ligand and receptor will bind to each other.

4. Collect the residue mixture in a flask.

5. Elute out the receptor from column from receptor ligand complex using elution buffer.

Please rate high.