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Please explain step-by-step. I'm totally lost here!
thanks in advance.
To determine the kinetic characteristics of an enzyme you used 1 nmol/L of enzyme in a series of assays where you measured th

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Answer #1

The plot of v vs. s is shown below

V vs. [s] curve v/ Lumol/L/min 50 200 250 100 150 [s] / umol/L

We know from michadi menten equation va Vmax [571 -Km 4 cs] Km is the substrate oncenhationat Which the rate is half of Vmax.This Vimax & [5] Km+CS] Vmaxx 200 mm (20+200) uh 150 uh um.min. Vmax = 150 x 220 200 loma l = 165 umoliti mint = 165 um/min INow Consider Vinear 165 um/min Vmax CS km tes] Since Vmax = 165 mm/min, the Km Should have numerical value where the substratKeat & Vinax 67. - Where CET is the initial concentration of enzynne. Inmol 163 umor DE. - Innoy = 105 umol/L Keat = 165 umbl

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    To determine the kinetic characteristics of an enzyme you used 1 nmol/L of enzyme in a series of assays where you measured the rate of reactions as you varied the concentration of substrate in each assay (Table A). Estimate from a Michaelis-Menten plot approximate values for Vmax, KM, Kcat, and the specificity constant for this enzyme and substrate. (The only information that is given is this paragraph and the table below). Table 1: [S] (μM) v (μmol/L/min) 0 0 5...

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