Question

In HPLC, if the particle size is reduced from 3.17 um to 1.19 um but all other conditions remain constant, by what factor will the column pressure need to be increased to maintain a constant linear flow rate? factor Alternatively, if the column pressure is increased by a factor of 10.0 but all other conditions remain constant, by what factor will the linear flow rate increase? factor

Answer 1

In HPLC ( high Performance Liquid Chromatography) the relation between column pressure and particle size is inversly proportional while the column pressure and flow rate is directly proportional.

The detail relation and the solution is shown below.

In HPLC, the column pressure is inversely proportional to the square of the particle size diameter (4) thens. column pressure, & - k [ka constant (1) reduced from d = 3.14 um the particle size is to d₂ = 1.19 cm. P2 = 13.172) 5 1P2 = 7.096p, so, the coloum pressure, P2, should be 7.09691 to maintain a constant linear flow pate. (2) coloum pressure (e is directly proportional to the lineap flow rate (0) while all other factors pomain Constant. p -ko. is 12 = 100; then, so, How rate will be value. 2 = 10 10 time higher than initial