Question

In studying a particular biomolecule (a protein, nucleic acid, carbohydrate, or lipid) in the laboratory, the biochemist first needs to separate it from other biomolecules in the sample (that is, it needs to be purified). Specific purification techniques are described later in the text. However, by looking at the monomeric subunits and functional groups of a biomolecule, you should have some ideas about the characteristics of the molecule that would allow you to separate it from other molecules. Look for differences in ionizable groups, polarity, solubility in water, size, and shape. Based on the characteristics of each type of molecule, select the way(s) in which amino acids such as alanine could be separated from fatty acids such as palmitate.

In studying a particular biomolecule (a protein, nucleic acid, carbohydrate, or lipid) in the laboratory, theapo biochemist first needs to separate it from other biomolecules in the sample (that is, it needs to be purifiea Specific purification techniques are described later in the text. However, by looking at the monomeric subunits and functional groups of a biomolecule, you should have some ideas about the characteristics of the molecule that would allow you to separate it from other molecules. Look for differences in ionizable groups, polarity, solubility in water, size, and shape. Based on the characteristics of each type of molecule, select the way(s) in which amino acids such as alanine could be separated from fatty acids such as palmitate. the presence of a negatively charged group on one molecule the solubilities of the two molecules in water □ the presence of a positively charged group on one molecule COO H3NH Scroll down to complete this question. 0

Answer 1

answer- seperation of amino acid (alanine) for fatty acid (palmitate) can be done by pressence of a positively charged group on amino acids . because both alanine and fatty acid got carboxylic group and also hydrocarbon , in palimitate their is long chain of hydrocarbon and a carboxylic group , where as alanine has a methyl group attached to that carbon through which both carboxylic and amino group attached

alanine can exits in both postive and negative charge form compare to fatty acid which can exists as negative charged form due to pressence of carboxylic group

alanine is water soluble whereas palimitate is not water soluble due to presence of long saturated carbon chain

similarly uridine can exists in negatively charged form due to phosphate group wheras gulcose cannot exists in that form , so can seperate them due to pressence of a negatively charged group on uridine triphosphate.

solubility of uridine triphosphate and gulcose is almost same due to pressence of polar groups