Question

Put the following steps in order to generate a MM Graph. Some steps may be unnecessary.

Question6 3 pts Match the following statements with the BEST choices- there might be some answers that do not match to anything. At [S Km.. [ Choose ] MM kinetics do not apply, since its assumptions are violated Increases Km Increases vO of the reaction at a given [S] Reaction is at steady state Most of the substrate is in the E.TS state Half of the enzyme molecules are bound to substrate [Choose] When the reaction is at Keq... Increasing [E]

Answer 1

Michaelis Menten equation depicts the best known model for kinetics of enzyme catalyzed reaction.

Thus, at [S] = Km, Vo will be equal to half of Vmax i.e, half of the enzyme molecules are bound to substrate molecule.

2 Km MM Cra.oh

When the reaction is at Keq, it is at steady state where rate of formation of Enzyme Substrate (ES) complex is equal to the rate of is equal to the rate of decomposition of ES.

Increasing [E], will affect Vmax of a reaction at a given substrate concentration. But is not applicable to MM kinetics as in MM equation it is assumed that [S] >> [E] .