Question

Which one or more of the four hierarchical levels of protein structure is/are always lost during protein denaturation?

* I know the answer is tertiary but want to know why?

My uni gave me this table below but I don't know why the answer is then teriary(they gave this answer). Can someone please explain.

*

On what structural features does the catalytic activity of an enzyme depend? (1) Definition:"Enzyme denaturation" loss of enzyme structure, (almost) always with loss of biological function Treatment Protein structure level(s) still intact? Biological function 1° 2 3" 4 if relevant retained? none (untreated enzyme) subunit dissociation (if relevant) eg. gentle detergent treatment X ? unfolding of protein eg. urea, moderate heat, moderate ApH X X X X X XX complete peptide bond hydrolysis (eg. 6M HCl and boiling overnight) X X

Answer 1

Sime, it is tertiam stuchase that aluoays lost okin structure dusina denaturafion To undestand this activib dimentional structune dhat made this structure 4enerally this tertiam stucture stabilise b weak mon- and disulfide bo end which ave easiho degr adef ho mil detergent activi dhe ofhe Seconcang amd primm stnettue stabilisedd (ebticde, H-bond,tone bnd) bshons heed to umderstand the three we cayalemt intesactin dclergent actvi