Question

1) Regarding the ALU element in the TPA-25 gene, are vou homozygous for the Alu insert, heterozygous, or Alu insert null? 2) Based off of your genotypes, what conclusions can you make about your biological parent's genotype? 3) Why do you think there are different genotypes associated with Alu elements.. ie Why doesn't everyone have the number of Alu inserts? 4) What could be a negative consequence of Alu insertions?

Answer 1

1:- in the experiment, oligonucleotide primers flanking the insertion site will be used to amplify a segment of the TPA gene. The expected product of the PCR reaction will be a 400-bp fragment when TPA-25 is present and 100-bp fragment when it is absent each of the three possible genotypes-homozygous for presence of TPA-25 (400-bp fragment only), homozygous for the absence of TPA-25(100bp fragment only) and heterozygous (400-bp and 100-bp fragment) -will be able to the determined following electrophoresis of our PCR reaction products in an agarose gel

2:and each parent has a homozygous genotype. Using the parents genotype each inner square is filled with a possible genotype for their child. All possible genotype of their children have a freckle-dominant allele , predicting a 100% chance of their children having freckles

4: alu insertiots are sometimes disruptive and can result in inherited disorders in the human genome, the mostly recently active have been the 22Alu Y and 6Alu