Problem

The following polypeptide was hydrolyzed by trypsin:Gly-Ser-Asp-Ala-Leu-Pro-Gly-Ile-Thr-Se...

The following polypeptide was hydrolyzed by trypsin:

Gly-Ser-Asp-Ala-Leu-Pro-Gly-Ile-Thr-Ser-Arg-Asp-Val-Ser-Lys-Val-Glu−

Tyr-Phe-Glu-Ala-Gly-Arg-Ser-Glu-Phe-Lys-GluPro-Arg-Leu-Tyr-

Met-Lys-Val-Glu-Gly-Arg-Pro-Val-Ser-Ala-Gly-Leu-Trp

a. How many fragments are obtained from the peptide?


b. In what order would the fragments be eluted from an anion-exchange column using a buffer of pH = 5?

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