Determine the primary structure of an octapeptide from the following data:
Acid hydrolysis gives 2 Arg, Leu, Lys, Met, Phe, Ser, Tyr.
Carboxypeptidase A releases Ser.
Edman’s reagent releases Leu.
Cyanogen bromide forms two peptides with the following amino acid compositions:
1. Arg, Phe, Ser
2. Arg, Leu, Lys, Met, Tyr Trypsin forms the following two amino acids and two peptides:
3 Arg
4 Ser
5 Arg, Met, Phe
6 Leu, Lys, Tyr
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