The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows:
[S] (μmol/L) | v [(μmol/L) min−1] |
5 | 22 |
10 | 39 |
20 | 65 |
50 | 102 |
100 | 120 |
200 | 135 |
(a) Estimate Vmax and KMfrom a direct graph of v versus [S] (these data are plotted in Figure 11.24b). Do you find difficulties in getting clear answers?
(b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better?
(c) Finally, try an Eadie-Hofstee plot of the same data.
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