Quantitative relationships between rate constants to calculate Km, kinetic efficiency (kcat/Km) and Vmax –V
Acetylcholinesterase catalyzes the hydrolysis of the neurotransmitter acetylcholine:
Acetylcholine + H2O→ acetate + choline
The Km of acetylcholinesterase for its substrate acetylcholine is 9 x 10-5 M. In a reaction mixture containing 5 nanomoles/mL of acetylcholinesterase and 150 μM acetylcholine, a velocity v0 = 40 μmol/mL·sec was observed for the acetylcholinesterase reaction.
a. Calculate the Vmax for this amount of enzyme.
b. Calculate kcat for acetylcholinesterase.
c. Calculate the catalytic efficiency (kcat/Km) for acetylcholinesterase.
d. Does acetylcholinesterase approach “catalytic perfection”?
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