Quantitative relationships between rate constants to calculate Km, kinetic efficiency (kcat/Km) and Vmax –IV
Carbonic anhydrase catalyzes the hydration of CO2.
CO2 + H2O⇌ H2CO3
The Km of carbonic anhydrase for CO2 is 12 mM. Carbonic anhydrase gave an initial velocity v0 = 4.5 µmols H2CO3 formed/mL sec when [CO2] = 36 mM.
a. What is the Vmax for this enzyme?
b. Assuming 5 pmol/mL (5 x 10-12 moles/mL) of enzyme were used in this experiment, what is kcat for this enzyme?
c. What is the catalytic efficiency of this enzyme?
d. Does carbonic anhydrase approach “catalytic perfection”?
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