Graphing the results from kinetics experiments with enzyme inhibitors
The following kinetic data were obtained for an enzyme in the absence of inhibitor (1), and in the presence of two different inhibitors (2) and (3) at 5 mM concentration. Assume [ET] is the same in each experiment.
[S] | (1) | (2) | (3) |
(mM) | v(µmol/mL·sec | v (µmol/mL·sec | v(µmol/mL·sec |
1 | 12 | 4.3 | 5.5 |
2 | 20 | 8 | 9 |
4 | 29 | 14 | 13 |
8 | 35 | 21 | 16 |
12 | 40 | 26 | 18 |
Plot these data as double-reciprocal Lineweaver-Burk plots and use your graph to answers a. and b.
a. Determine Vmax and Km for the enzyme.
b. Determine the type of inhibition and the KI for each inhibitor.
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