Draw substrate saturation curves for the substrates of CTP synthetase which show both positive and negative cooperativity
CTP synthetase catalyzes the synthesis of CTP from UTP:
UTP + ATP + glutamine ⇌ CTP + glutamate + ADP + Pi
The substrates UTP and ATP show positive cooperativity in their binding to the enzyme, which is anα4-type homotetramer. However, the other substrate, glutamine, shows negative cooperativity. Draw substrate saturation curves of the form v versus [S]/K0.5 for each of these three substrates that illustrate these effects.
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