Problem

Determining the Primary Structure of a ProteinREFLECT AND APPLY Which enzymes or chemicals...

Determining the Primary Structure of a Protein

REFLECT AND APPLY Which enzymes or chemicals would you choose to use to cut the protein from Question? Why?

Question

REFLECT AND APPLY Assume that you are getting ready to do an amino acid sequencing experiment on a protein containing 100 amino acids, and amino acid analysis shows the following data:

Amino Acid

Number of Residues

Ala

7

Arg

23.7

Asn

5.6

Asp

4.1

Cys

4.7

Gln

4.5

Glu

2.2

Gly

3.7

His

3.7

Ile

1.1

Leu

1.7

Lys

11.4

Met

0

Phe

2.4

Pro

4.5

Ser

8.2

Thr

4.7

Trp

0

Tyr

2.0

Val

5.1

Which of the chemicals or enzymes normally used for cutting proteins into fragments would be the least useful to you?

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