Determining the Primary Structure of a Protein
REFLECT AND APPLY With which amino acid sequences would chymotrypsin be an effective reagent for sequencing the protein from Question? Why?
Question
REFLECT AND APPLY Assume that you are getting ready to do an amino acid sequencing experiment on a protein containing 100 amino acids, and amino acid analysis shows the following data:
Amino Acid | Number of Residues |
Ala | 7 |
Arg | 23.7 |
Asn | 5.6 |
Asp | 4.1 |
Cys | 4.7 |
Gln | 4.5 |
Glu | 2.2 |
Gly | 3.7 |
His | 3.7 |
Ile | 1.1 |
Leu | 1.7 |
Lys | 11.4 |
Met | 0 |
Phe | 2.4 |
Pro | 4.5 |
Ser | 8.2 |
Thr | 4.7 |
Trp | 0 |
Tyr | 2.0 |
Val | 5.1 |
Which of the chemicals or enzymes normally used for cutting proteins into fragments would be the least useful to you?
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