Extracting Pure Proteins from Cells
REFLECT AND APPLY You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate precipitation as the next step. Knowing nothing beforehand about the amount of ammonium sulfate to add, design an experiment to find the proper concentration (% saturation) of ammonium sulfate to use.
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