Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems: (a) Calculate the pH of the tris buffer after mixing 15.0 mL of 0.10 M HCl solution with 25.0 mL of 0.10 M tris. (b) This buffer was used to study an enzyme-catalyzed reaction. As a result of the reaction, 0.00015 mole of H+ was consumed. What is the pH of the buffer at the end of the reaction? (c) What would be the final pH if no buffer were present?
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