1. Using Excel, make a Michaelis-Menten plot from the following data. Properly label the axes with...
14. (The three sentences minimum rule will not be applicable to the following items) Determine the values of KM and Vmax for the decarboxylation of a B-keto acid given the following data. Substrate Concentration (mol L') Velocity (mM min') 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256
(I need help with part C, Drawing the expected Michaelis-Menten plot; Do NOT draw the Lineweaver-Burk plot. thanks!) 1. Michaelis-Menten kinetics- use the M-M equation to answer the following: a. An enzyme (5 µM) has a Vmax of 450 mM/min. What is kcat? b. When the substrate concentration is 50 mM, the initial velocity (V0) was measured to be 375 mM/min. Under the conditions described above, calculate the KM. c. Draw the expected Michaelis-Menten plot (label your axes and include...
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel: On the M-M Plot: estimate Vmax, KM On the L-B Plot: determine Vmax, KM, keat, kcat/Km. The total enzyme concentration is 5 uM. Graphs can be 1/2 page. Must be computer generated with all axes labeled. Substrate (mM) V. (mM/s) | 1/[S] (mM1) 1/V. (s/mM) 10 | 0 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228...
Michaelis-Menten plot and Lineweaver-Burk plot calculations- use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel to determine Vinas, KM, kcat, kcat/KM. The total enzyme concentration is 5 μM. Graphs can be 2 page. Must be computer generated with all axes labeled. Substrate (mM Vo (mM/s) 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228 0.303
5. The rate of a simple enzyme reaction is given by the standard Michaelis-Menten equation. a. If the Vmax = 354 umol/sec and the KM = 1.5 mm, at what substrate concentration is the rate is 177 umol/sec? b. Using MATLAB, plot a graph of rate versus substrate concentration using the enzyme parameters in part (a) for (S] = 0.5 to 30.0 mM in increments of 0.5 mM). Don't forget to include units on your axes. Include a copy of...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
thank you a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL Velocity umole/(min-mL) 10 15 20 31 50 75 100 125 250 330 380 437 524 612 662 678 696 713 Have the substrate concentration as the x-axis of the graph, and the velocity as the y-axis. . Use a grid line every 10 substrate concentration units, and a grid line every 20 velocity units. . In Excel, gridlines can be controlled under the Chart...
i need help with all question 5 please! thank you Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...