Homework Answers
In the analytical chemistry, the calibration curve, which also called a standard curve, is a general technique for finding the concentration of a compound present in an unknown sample by comparing that unknown to some set of standard samples of known concentration.
The calibration curve is a curve plotted between instrumental response, and the analytical signal, which generally changes with the concentration of the analyte present.
It is generally straight line. And straight line needs only two point yo form. But to get an accurate result and to make sure they form a straight line we take five measurements.
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Questions 1. The calibration curve is a straight line. Since only two points are required to...
Question 1: When creating the calibration curve during week 1, it was determined that [KSCN] =...
Question 1: When creating the calibration curve during week 1, it was determined that [KSCN] = [FeSCN2+] at equilibrium. Explain why these two concentrations were equal in terms of the relative concentrations of each reactant, the limiting reactant, and the direction in which the reaction proceeded. Question 2: Describe the process by which equilibrium concentrations of the reactants and product were determined. Include in your discussion, how the equilibrium (final) concentration of FeSCN2+ was determined using a calibration curve, mole-to-mole...
The data in the table are used to create a calibration curve for the determination of...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (μg Absorbance at 260 nm 0.00 0.232 10.63 0.535 21.26 0.808 31.89 1.077 42.52 1.355 m= ? in μg−1 b= Using the...
What is the minimum number of samples that could make a standard line on the calibration curve and why do we use many sa...
What is the minimum number of samples that could make a standard line on the calibration curve and why do we use many samples
To construct a calibration curve we would need to take our stock solution and dilute it...
To construct a calibration curve we would need to take our stock solution and dilute it to different concentrations. We would then test those different concentration in our spectrophotometer to test their absorbance. If we diluted our samples as follows, calculate the resulting concentrations. (HINT: this is dilution which means we can use M1V1=M2V2, where M1 is our original 150 mM concentration, V1 is the volume of the original concentration that we added water to dilute, M2 is the concentration...
Question 6 6 points Save Answe You construct a calibration curve for conductivity of Na2HPO4 solutions...
Question 6 6 points Save Answe You construct a calibration curve for conductivity of Na2HPO4 solutions with concentration (M) on the x axis and conductivity (ms) on the y-axis. You have a sample of Na2HPO4 that you want to determine the concentration of using this curve. You measure its conductivity to be 3.93 ms. The equation for the line from your calibration curve is y = 138 mS/M X +0.25 ms. Use this information to determine the concentration of your...
1. We will be building a Cu? and Zn galvanic cell. Write the two half reactions...
1. We will be building a Cu? and Zn galvanic cell. Write the two half reactions (one as a reduction and one as an oxidation based on forming a spontaneous reaction) and write the combined reaction. (Similar to equations 9.3-9.5 but for our system specifically). (3 pts) In the galvanic cell we are building in class, which is the anode and which is the cathode? (2 pts) 2. Calculate Ecel for our voltaic cell. (Use Table 16.1 in your OpenStax...
Question 1: When creating the calibration curve during week 1, it was determined that [KSCN] = [...
Question 1: When creating the calibration curve during week 1, it was determined that [KSCN] = [FeSCN2+] at equilibrium. Explain why these two concentrations were equal in terms of the relative concentrations of each reactant, the limiting reactant, and the direction in which the reaction proceeded Question 2: Describe the process by which equilibrium concentrations of the reactants and product were determined. Include in your discussion, how the equilibrium (final) concentration of FeSCN2+ was determined using a calibration curve, mole-to-mole...
help! i'm not sure how to go about this. ABSORBANCE PLOT DATA Please attach a copy...
help! i'm not sure how to go about this.
ABSORBANCE PLOT DATA Please attach a copy of your print out from the spectrophotometer, or indicate that it is attached to your lab partners report and give their name. ron A= emll Analytical wavelength used (=(A-b)lum CALIBRATION CURVE DATA Concentration of Stock Solution: 5.00 X 10 M Door Test Tube Volume Final Volume Final Stock Soln. Solution Concentration 1 2.00 10.0 Absorbance Trial 1 Absorbance Trial 2 2. 4.00 10.0 3...
1. Why converting transmittance to absorbance for IR sucrose calibration curve? 2. Explain two reasons for...
1. Why converting transmittance to absorbance for IR sucrose calibration curve? 2. Explain two reasons for the differences between the IR spectra of solid sucrose and 24wt% aqueous citingspecific wavenumbers where applicable. Hints: background and hydrolysis
III. Standard Curves When we want to determine the concentration of an unknown solution, we typically measure a certain...
III. Standard Curves When we want to determine the concentration of an unknown solution, we typically measure a certain property of standard solutions with our standard (known) solutions and create what's called a standard curve (or calibration curve). A standard curve relates two variables of certain known solutions. That way, when there is an unknown variable for a certain solution, it can be determined through the standard curve. When plotting standard curves, we always plot the independent variable on the...
Question 6 6 points Save Answe You construct a calibration curve for conductivity of Na2HPO4 solutions with concentration (M) on the x axis and conductivity (ms) on the y-axis. You have a sample of Na2HPO4 that you want to determine the concentration of using this curve. You measure its conductivity to be 3.93 ms. The equation for the line from your calibration curve is y = 138 mS/M X +0.25 ms. Use this information to determine the concentration of your...
1. We will be building a Cu? and Zn galvanic cell. Write the two half reactions (one as a reduction and one as an oxidation based on forming a spontaneous reaction) and write the combined reaction. (Similar to equations 9.3-9.5 but for our system specifically). (3 pts) In the galvanic cell we are building in class, which is the anode and which is the cathode? (2 pts) 2. Calculate Ecel for our voltaic cell. (Use Table 16.1 in your OpenStax...
help! i'm not sure how to go about this.
ABSORBANCE PLOT DATA Please attach a copy of your print out from the spectrophotometer, or indicate that it is attached to your lab partners report and give their name. ron A= emll Analytical wavelength used (=(A-b)lum CALIBRATION CURVE DATA Concentration of Stock Solution: 5.00 X 10 M Door Test Tube Volume Final Volume Final Stock Soln. Solution Concentration 1 2.00 10.0 Absorbance Trial 1 Absorbance Trial 2 2. 4.00 10.0 3...
III. Standard Curves When we want to determine the concentration of an unknown solution, we typically measure a certain property of standard solutions with our standard (known) solutions and create what's called a standard curve (or calibration curve). A standard curve relates two variables of certain known solutions. That way, when there is an unknown variable for a certain solution, it can be determined through the standard curve. When plotting standard curves, we always plot the independent variable on the...
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