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A peak elutes from an HPLC column 17.3 cm in length in 14.0 min. What would...
A peak elutes from an HPLC column 11.8-cm in length in 13.8 min. What would be...
A peak elutes from an HPLC column 11.8-cm in length in 13.8 min. What would be the width at half-height of the peak (in seconds) if the plate height were: (a) 6.9 μm? (b) 36 μm?
12. In a HPLC chromatogram, peak A elutes at 2.5 min and peak B elutes at...
12. In a HPLC chromatogram, peak A elutes at 2.5 min and peak B elutes at 3.0 min. The width of peak A is 0.8 min and peak B is 0.6 min. What is the resolution? Are these two peaks baseline resolved (explain your reasoning)? If not, change a specific parameter that would make these two peaks baseline resolved and calculate new resolution. Diagram both chromatograms (original question and baseline resolved) (8 pts)
A HPLC column was operated under the following conditions Column Length – 25.4 cm Column Diameter...
A HPLC column was operated under the following conditions
Column Length – 25.4 cm Column
Diameter = 0.5 mm
Mass stationary phase = 0.49
g Density
Stationary Phase = 1.12 g/mL
Measured Outlet Flow Rate = 1.0 mL/min
Column Temperature = 25 oC.
resolution equation=Rs = Trb-Tra/W
Plate height(H) = length of column (L)/Number of plate on the
column (N)
No. of theoretical N= 16(RS/w)2
Calculate the resolution for components #3 and #4?
Calculate the plate height for column (use...
Suppose that an HPLC column produces Gaussian peaks. The detector measures absorbance at 254 nm. A...
Suppose that an HPLC column produces Gaussian peaks. The detector measures absorbance at 254 nm. A sample containing equal moles of A and B was injected into the column. Compound A (€254 = 3.21 x 104 M-1 cm1) has a height of 150. mm and a half width W1/2 = 12.5 mm. Compound B (€254 = 1.51 x 104 m 1 cm-1) has w1/2 = 8.5 mm. The height of peak B is mm.
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min...
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
A chromatogram gives an ideal Gaussian peak with tr=9.10 min and w1/2=0.15 min. How many theoretical...
A chromatogram gives an ideal Gaussian peak with tr=9.10 min and w1/2=0.15 min. How many theoretical plates does the column have? N= plates What is the plate height of the column in millimeters if the column is 12.1 cm long? H=
A chromatogram gives an ideal Gaussian peak with tr = 9.80 min and w1/2 = 0.17...
A chromatogram gives an ideal Gaussian peak with tr = 9.80 min and w1/2 = 0.17 min. How many theoretical plates does the column have? N = plates What is the plate height of the column in millimeters if the column is 10.8 cm long? H =
A chromatogram gives an ideal Gaussian peak with t; = 8.50 min and w1/2 = 0.20...
A chromatogram gives an ideal Gaussian peak with t; = 8.50 min and w1/2 = 0.20 min. How many theoretical plates does the column have? N N=1 = plates What is the plate height of the column in millimeters if the column is 11.1 cm long? H = mm
What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
Two compounds C and D are separated on a column 34 cm long . Given the...
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
12. In a HPLC chromatogram, peak A elutes at 2.5 min and peak B elutes at 3.0 min. The width of peak A is 0.8 min and peak B is 0.6 min. What is the resolution? Are these two peaks baseline resolved (explain your reasoning)? If not, change a specific parameter that would make these two peaks baseline resolved and calculate new resolution. Diagram both chromatograms (original question and baseline resolved) (8 pts)
A HPLC column was operated under the following conditions
Column Length – 25.4 cm Column
Diameter = 0.5 mm
Mass stationary phase = 0.49
g Density
Stationary Phase = 1.12 g/mL
Measured Outlet Flow Rate = 1.0 mL/min
Column Temperature = 25 oC.
resolution equation=Rs = Trb-Tra/W
Plate height(H) = length of column (L)/Number of plate on the
column (N)
No. of theoretical N= 16(RS/w)2
Calculate the resolution for components #3 and #4?
Calculate the plate height for column (use...
Suppose that an HPLC column produces Gaussian peaks. The detector measures absorbance at 254 nm. A sample containing equal moles of A and B was injected into the column. Compound A (€254 = 3.21 x 104 M-1 cm1) has a height of 150. mm and a half width W1/2 = 12.5 mm. Compound B (€254 = 1.51 x 104 m 1 cm-1) has w1/2 = 8.5 mm. The height of peak B is mm.
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
A chromatogram gives an ideal Gaussian peak with tr = 9.80 min and w1/2 = 0.17 min. How many theoretical plates does the column have? N = plates What is the plate height of the column in millimeters if the column is 10.8 cm long? H =
A chromatogram gives an ideal Gaussian peak with t; = 8.50 min and w1/2 = 0.20 min. How many theoretical plates does the column have? N N=1 = plates What is the plate height of the column in millimeters if the column is 11.1 cm long? H = mm
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