What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
A peak elutes from an HPLC column 11.8-cm in length in 13.8 min. What would be the width at half-height of the peak (in seconds) if the plate height were: (a) 6.9 μm? (b) 36 μm?
A HPLC column was operated under the following conditions
Column Length – 25.4 cm Column
Diameter = 0.5 mm
Mass stationary phase = 0.49
g Density
Stationary Phase = 1.12 g/mL
Measured Outlet Flow Rate = 1.0 mL/min
Column Temperature = 25 oC.
resolution equation=Rs = Trb-Tra/W
Plate height(H) = length of column (L)/Number of plate on the
column (N)
No. of theoretical N= 16(RS/w)2
Calculate the resolution for components #3 and #4?
Calculate the plate height for column (use...
4) What is the order of elution of the following compounds from a reverse-phase packed HPLC column? Briefly justify your reasoning. Benzyl alcohol (C6H5CH2OH), benzaldehyde (C6H5CHO), benzoic acid (C6H5COOH), Acetophenone (C6H5C(O)CH3)
A peak elutes from an HPLC column 17.3 cm in length in 14.0 min. What would be the width at half-height of the peak (in seconds) if the plate height were 4.83 um? W1/2 = What would be the width at half-height of the peak (in seconds) if the plate height were 39.0 um? W1/2 =
3. (2 pts) Why is eddy diffusion a larger contribution to plate height in packed column gas chromatography than in liquid chromatography?
When packing a column, normally to about what height should the column be packed with adsorbent? A. to a height equal to one-fourth the mass of the solvent B. to a height about 10 times the diameter of the column being use C. about 4 centimeters below the top of the column D. about 18 centimeters up from the bottom
What particle diameter is needed to give 15 000 plates over a 10-cm HPLC column?
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed with Chromosorb P and Carbowax 20M as stationary phase. The following retention times were obtained: air: 18.0 sec; methylacetate: 2.98 min; methyl propionate: 4.56 min. Peak width at half height: methylacetate: 7.7 sec; methyl propionate: 12.4 sec. Calculate: a) the corrected retention time for each solute. b) the capacity factor k' for each solute. c) the selectivity factor α for the pair of solutes....
Question 1 Which of the following is the advantage of straight packed column? Selected Answer: It can be repacked easily Answers It can be packed uniformly It can be repacked easily It is compact It is easier to heat it evenly Question 2 Which of the following statements most accurately describes thin-layer chromatography? Selected Answer: technique used to separate non-volatile mixtures, the mobile phase has different properties from the stationary phase. Answers The mobile phase is the solvent also called...
A solute with a partition coefficient of 3.5 is extracted from 10.0 mL of phase 1 into phase 2. What volume of phase 2 (in mL) is required to extract 99% of the solute from phase 1 in a single extraction?