A HPLC column was operated under the following conditions
Column Length – 25.4 cm Column Diameter = 0.5 mm
Mass stationary phase = 0.49 g Density Stationary Phase = 1.12 g/mL
Measured Outlet Flow Rate = 1.0 mL/min
Column Temperature = 25 oC.
resolution equation=Rs = Trb-Tra/W
Plate height(H) = length of column (L)/Number of plate on the column (N)
No. of theoretical N= 16(RS/w)2
Calculate the resolution for components #3 and #4?
Calculate the plate height for column (use component #3?
Calculate the number of theoretical plates(use component #3?
Homework Answers
The resolution for components #3 = (2.9 - 1.2)/w
Note: Here, the width at half-height (w) values are not given, actually, if the peak length is given, then half-of the peak length itself is the value of 'w'.
The resolution for components #4 = (4.1 - 1.2)/w
Note: Here also, the width at half-height (w) values are not given, actually, if the peak length is given, then half-of the peak length itself is the value of 'w'.
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