Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture...
Length of column = 30.50 cm
Flow rate = 0.313 mL/min
A chromatogram of a mixture of species A, B, C, and D provided the following data:
|
Species |
Retention time, minutes |
Width of peak base (W), minutes |
|
A |
5.4 |
0.41 |
|
B |
13.3 |
1.07 |
|
C |
14.1 |
1.16 |
|
D |
21.6 |
1.72 |
Calculate the resolution for species C and D
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Length of column = 30.50 cm
Flow rate = 0.313 mL/min
A chromatogram of a mixture...
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The following data applies to a column for liquid chromatography: Use this information to answer the question below Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture of species A, B, C, and D provided the following data: Species Retention time, minutes Width of peak base (W), minutes А 5.4 0.41 B 13.3 1.07 C 14.1 1.16 D 21.6 1.72 Calculate the resolution for species C and D Select one: O A 2.60...
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help needed with this problem set
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The following data applies to a column for liquid chromatography: Use this information to answer the question below Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture of species A, B, C, and D provided the following data: Species Retention time, minutes Width of peak base (W), minutes А 5.4 0.41 B 13.3 1.07 C 14.1 1.16 D 21.6 1.72 Calculate the resolution for species C and D Select one: O A 2.60...
(3) During the separation of two steroids by HPLC on a C18 column, a student reported the following elution times with a certain % acetonitrile/water mobile phase (uracil was added to the steroid mixture) tm (uracil) 1.2 min t, (steroid1) 4.55 min t, (steroid2) 6.55 min base width, w (steroid 1) 0.56 min base width, w (steroid 2) 0.79 min a. Sketch the chromatogram to illustrate the above separation. Label the peaks and the x- and y-axis. Calculate the resolution...
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help needed with this problem set
Refer to the HPLC chromatograms below for a 5-component mixture of organic acids for the following questions. The specific HPLC parameters for chromatogram A (top) are described in the figure title and the insert. You only need the lecture notes and/or your personal experience to answer those questions. Mobile phase: 30 % MeCN, 70 % TFA, 0.1 M (pH 2.0) low rate: 1.0 mL/min 2Benzok Acid Maleic Aci 1. (a) Which component has the...
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