A peak elutes from an HPLC column 11.8-cm in length in 13.8 min. What would be the width at half-height of the peak (in seconds) if the plate height were: (a) 6.9 μm? (b) 36 μm?
A peak elutes from an HPLC column 11.8-cm in length in 13.8 min. What would be...
A peak elutes from an HPLC column 17.3 cm in length in 14.0 min. What would be the width at half-height of the peak (in seconds) if the plate height were 4.83 um? W1/2 = What would be the width at half-height of the peak (in seconds) if the plate height were 39.0 um? W1/2 =
12. In a HPLC chromatogram, peak A elutes at 2.5 min and peak B elutes at 3.0 min. The width of peak A is 0.8 min and peak B is 0.6 min. What is the resolution? Are these two peaks baseline resolved (explain your reasoning)? If not, change a specific parameter that would make these two peaks baseline resolved and calculate new resolution. Diagram both chromatograms (original question and baseline resolved) (8 pts)
What is the plate height for a 10.0-cm HPLC column packed with 3.5 μm solid phase?
A HPLC column was operated under the following conditions Column Length – 25.4 cm Column Diameter = 0.5 mm Mass stationary phase = 0.49 g Density Stationary Phase = 1.12 g/mL Measured Outlet Flow Rate = 1.0 mL/min Column Temperature = 25 oC. resolution equation=Rs = Trb-Tra/W Plate height(H) = length of column (L)/Number of plate on the column (N) No. of theoretical N= 16(RS/w)2 Calculate the resolution for components #3 and #4? Calculate the plate height for column (use...
3. a) b) c) How many theoretical plates produce a chromatography peak eluting at 4.78 min with a full width at half max (FWHM) of 1.20 s? The length of the column is 30.0 m. What is the plate height in microns? What is the peak resolution for this peak and another peak eluted at 4.73 minutes that has a full peak width (measured at the base) of 2.14 seconds? Is this sufficient for quantitative analysis of those two components?...
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
Suppose that an HPLC column produces Gaussian peaks. The detector measures absorbance at 254 nm. A sample containing equal moles of A and B was injected into the column. Compound A (€254 = 3.21 x 104 M-1 cm1) has a height of 150. mm and a half width W1/2 = 12.5 mm. Compound B (€254 = 1.51 x 104 m 1 cm-1) has w1/2 = 8.5 mm. The height of peak B is mm.
GIve me the answer in a correct one significant figure A chromatogram gives an ideal Gaussian peak with a retention time of 8.20 min and a peak width at half height of 0.16 min. If the column is 10.2 cm long then what is the plate height in mm (round your answer to one significant figure and don't include units in your answer)?
Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture of species A, B, C, and D provided the following data: Species Retention time, minutes Width of peak base (W), minutes A 5.4 0.41 B 13.3 1.07 C 14.1 1.16 D 21.6 1.72 Calculate the resolution for species C and D
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed with Chromosorb P and Carbowax 20M as stationary phase. The following retention times were obtained: air: 18.0 sec; methylacetate: 2.98 min; methyl propionate: 4.56 min. Peak width at half height: methylacetate: 7.7 sec; methyl propionate: 12.4 sec. Calculate: a) the corrected retention time for each solute. b) the capacity factor k' for each solute. c) the selectivity factor α for the pair of solutes....